FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3033740803> ?p ?o ?g. }

• W3033740803 abstract "Abstract Background and Aims Lupus nephritis (LN) is a frequent manifestation of Systemic Lupus Erythematosus (SLE). The therapeutic strategy relies on the result of kidney biopsy, which differentiates proliferative LN (PLN) from non-proliferative LN (NPLN). The analysis of the urinary peptidome has led to the identification of prognostic biomarkers in chronic kidney disease (CKD, namely the CKD273 classifier), or, as a liquid biopsy, for the diagnosis of glomerulonephritides. We verified whether urinary peptidomics could predict the severity of renal pathological injury and damage in LN. Method Urine samples, collected before kidney biopsy, were analyzed by capillary-electrophoresis coupled to mass-spectrometry (CE-MS). Urinary peptide profiles were compared between PLN and NPLN. Predictive values for chronic pathological lesions (glomerulosclerosis and interstitial fibrosis/tubular atrophy (IF/TA)), response to therapy and development of CKD were also assessed. Clinical characteristics, routine laboratory parameters and immunological SLE markers were collected at inclusion and prospectively for at least 24 months. Results We collected 100 urinary samples from patients with LN, forming a discovery (n=67) and an independent validation (n=33) cohort. Overall there were 69 patients with PLN (class III or IV +/-V with active lesions) and 31 patients with NPLN (class II or V or chronic lesions). In the discovery cohort, the abundance of 36 urinary peptides differed between PLN and NPLN (Mann-Whitney test). However, these peptides did not resist multiple testing correction. Among them, 17 could be sequenced (fragments of collagen-I, -II, -III, apolipoprotein A-I, Fibrinogen alpha chain and Histone H2B). Of the 17 sequenced peptides, 7 were also part of the CKD273 classifier. A mathematical model combining the 36 peptides classified PLN and NPLN patients from the validation cohort with an AUC of 0.75 and sensitivity and specificity of 0.81 and 0.53, respectively. Positivity of anti-dsDNA antibodies had the best sensitivity (0.96) and sterile pyuria the best specificity (0.68) to differentiate PLN from NPLN, and including the CKD273 score did not improve the accuracy of the predictive models. No urinary peptidomics profile was identified to predict early response to therapy in patients with LN. Glomerulosclerosis was observed in 63/100 biopsies. In the discovery cohort, the abundance of 38 urinary peptides (including 22 sequenced) differed between patients with vs without glomerulosclerosis (Mann-Whitney test). Again, these peptides did not resist multiple testing correction. Of the 22 sequenced peptides, 7 (different from the peptides associated with PLN) belonged to the CKD273 classifier. A mathematical model combining the 38 peptides classified patients from the validation cohort with an AUC of 0.75 and sensitivity and specificity of 0.82 and 0.54, respectively, for the presence of glomerulosclerosis. Although the quantification of IF/TA was correlated to the pre-existing CKD273 classifier, including the CKD273 score did not improve the prediction of IF/TA when tested in combination with simple clinical parameters. Only 3 patients developed CKD after a mean follow-up of 4 years, therefore contribution of urinary peptidomics for the prediction of CKD in LN could not be evaluated. Conclusion Different urinary peptidomics signatures were identified among patients with LN, according to the presence of active proliferative lesions or chronic lesions. However, these panels did not resist multiple testing correction, and did not improve the diagnostic accuracy when combined with clinical or immunological markers. The contribution of urinary peptidomics to predict CKD in patients with LN, or as an early predictor of renal flares, could not be evaluated. Kidney biopsy remains central for the care of patients with LN." @default.
• W3033740803 created "2020-06-12" @default.
• W3033740803 creator A5018755487 @default.
• W3033740803 creator A5030594603 @default.
• W3033740803 creator A5030863027 @default.
• W3033740803 creator A5032149927 @default.
• W3033740803 creator A5040540371 @default.
• W3033740803 creator A5042474261 @default.
• W3033740803 creator A5048525538 @default.
• W3033740803 creator A5052974303 @default.
• W3033740803 creator A5063371313 @default.
• W3033740803 creator A5070924699 @default.
• W3033740803 creator A5072337161 @default.
• W3033740803 creator A5074798255 @default.
• W3033740803 creator A5078830017 @default.
• W3033740803 creator A5085831233 @default.
• W3033740803 date "2020-06-01" @default.
• W3033740803 modified "2023-10-02" @default.
• W3033740803 title "P0360URINARY PEPTIDOMIC ANALYSIS IN PROLIFERATIVE VERSUS NON-PROLIFERATIVE LUPUS NEPHRITIS : RESULTS OF THE PEPTIDU-LUP STUDY" @default.
• W3033740803 doi "https://doi.org/10.1093/ndt/gfaa142.p0360" @default.
• W3033740803 hasPublicationYear "2020" @default.
• W3033740803 type Work @default.
• W3033740803 sameAs 3033740803 @default.
• W3033740803 citedByCount "0" @default.
• W3033740803 crossrefType "journal-article" @default.
• W3033740803 hasAuthorship W3033740803A5018755487 @default.
• W3033740803 hasAuthorship W3033740803A5030594603 @default.
• W3033740803 hasAuthorship W3033740803A5030863027 @default.
• W3033740803 hasAuthorship W3033740803A5032149927 @default.
• W3033740803 hasAuthorship W3033740803A5040540371 @default.
• W3033740803 hasAuthorship W3033740803A5042474261 @default.
• W3033740803 hasAuthorship W3033740803A5048525538 @default.
• W3033740803 hasAuthorship W3033740803A5052974303 @default.
• W3033740803 hasAuthorship W3033740803A5063371313 @default.
• W3033740803 hasAuthorship W3033740803A5070924699 @default.
• W3033740803 hasAuthorship W3033740803A5072337161 @default.
• W3033740803 hasAuthorship W3033740803A5074798255 @default.
• W3033740803 hasAuthorship W3033740803A5078830017 @default.
• W3033740803 hasAuthorship W3033740803A5085831233 @default.
• W3033740803 hasBestOaLocation W30337408031 @default.
• W3033740803 hasConcept C126322002 @default.
• W3033740803 hasConcept C142724271 @default.
• W3033740803 hasConcept C2775934546 @default.
• W3033740803 hasConcept C2778653478 @default.
• W3033740803 hasConcept C2779134260 @default.
• W3033740803 hasConcept C2779561371 @default.
• W3033740803 hasConcept C2779912601 @default.
• W3033740803 hasConcept C2780091579 @default.
• W3033740803 hasConcept C2781087799 @default.
• W3033740803 hasConcept C71924100 @default.
• W3033740803 hasConcept C77411442 @default.
• W3033740803 hasConcept C90924648 @default.
• W3033740803 hasConceptScore W3033740803C126322002 @default.
• W3033740803 hasConceptScore W3033740803C142724271 @default.
• W3033740803 hasConceptScore W3033740803C2775934546 @default.
• W3033740803 hasConceptScore W3033740803C2778653478 @default.
• W3033740803 hasConceptScore W3033740803C2779134260 @default.
• W3033740803 hasConceptScore W3033740803C2779561371 @default.
• W3033740803 hasConceptScore W3033740803C2779912601 @default.
• W3033740803 hasConceptScore W3033740803C2780091579 @default.
• W3033740803 hasConceptScore W3033740803C2781087799 @default.
• W3033740803 hasConceptScore W3033740803C71924100 @default.
• W3033740803 hasConceptScore W3033740803C77411442 @default.
• W3033740803 hasConceptScore W3033740803C90924648 @default.
• W3033740803 hasLocation W30337408031 @default.
• W3033740803 hasOpenAccess W3033740803 @default.
• W3033740803 hasPrimaryLocation W30337408031 @default.
• W3033740803 hasRelatedWork W11221851 @default.
• W3033740803 hasRelatedWork W13927973 @default.
• W3033740803 hasRelatedWork W16884040 @default.
• W3033740803 hasRelatedWork W18264973 @default.
• W3033740803 hasRelatedWork W2748689 @default.
• W3033740803 hasRelatedWork W2972987 @default.
• W3033740803 hasRelatedWork W3451813 @default.
• W3033740803 hasRelatedWork W475345 @default.
• W3033740803 hasRelatedWork W5689981 @default.
• W3033740803 hasRelatedWork W8916055 @default.
• W3033740803 isParatext "false" @default.
• W3033740803 isRetracted "false" @default.
• W3033740803 magId "3033740803" @default.
• W3033740803 workType "article" @default.