Matches in SemOpenAlex for { <https://semopenalex.org/work/W3033876676> ?p ?o ?g. }
- W3033876676 endingPage "121" @default.
- W3033876676 startingPage "113" @default.
- W3033876676 abstract "Background: Esterase plays a major role in the degradation of natural materials, industrial pollutants and also provides an immense contribution to the eco-friendly approaches in various industrial applications. Objective: In the present study, extracellular esterase from bacterial isolate Bacillus licheniformis was purified, characterized and used in the synthesis of octyl acetate. Methods: Purification of esterase from Bacillus licheniformis was achieved using Sephadex G-75 column chromatography. Gas chromatography was used to analyze the octyl acetate synthesis. Results: The enzyme was salted out using ammonium sulphate precipitation and 60-70% saturation gave maximum specific activity of the enzyme during precipitation. A purification fold of 6.46 and yield of 9.69% was achieved when esterase from Bacillus licheniformis was purified using Sephadex G-75 column chromatography. Native as well as SDS-PAGE analysis gave a single band of 42 kDa. This showed that the enzyme was purified to homogeneity and it was a monomer with molecular weight of 42 kDa. Biochemical characterization of the enzyme revealed that it had optimum temperature of 45°C in 0.1 M Tris-HCl buffer of pH 8.0. On optimizing different parameters, such as molar ratio of reactants, incubation time, temperature, and amount of protein, the % yield of octyl acetate was found to be 77.3%. Conclusion: In this work, simple method was used to purify esterase and the enzyme was further used in producing esters/products of commercial value within a reasonably short period of 12 h with a maximum yield of 77.3%." @default.
- W3033876676 created "2020-06-12" @default.
- W3033876676 creator A5001193473 @default.
- W3033876676 creator A5024850253 @default.
- W3033876676 creator A5065482785 @default.
- W3033876676 creator A5068143361 @default.
- W3033876676 creator A5090109619 @default.
- W3033876676 date "2020-06-05" @default.
- W3033876676 modified "2023-09-25" @default.
- W3033876676 title "Purification and Properties of an Esterase from Bacillus licheniformis and it’s Application in Synthesis of Octyl Acetate" @default.
- W3033876676 cites W1966057026 @default.
- W3033876676 cites W1967103687 @default.
- W3033876676 cites W1982038711 @default.
- W3033876676 cites W1985744610 @default.
- W3033876676 cites W2000511735 @default.
- W3033876676 cites W2028404273 @default.
- W3033876676 cites W2048818535 @default.
- W3033876676 cites W2062631723 @default.
- W3033876676 cites W2075132966 @default.
- W3033876676 cites W2077054364 @default.
- W3033876676 cites W2098893421 @default.
- W3033876676 cites W2127478832 @default.
- W3033876676 cites W2133231167 @default.
- W3033876676 cites W2137139106 @default.
- W3033876676 cites W2160655600 @default.
- W3033876676 cites W2166537396 @default.
- W3033876676 cites W2296490069 @default.
- W3033876676 cites W2336227052 @default.
- W3033876676 cites W2586956401 @default.
- W3033876676 cites W2591380887 @default.
- W3033876676 cites W2600271788 @default.
- W3033876676 cites W2621337343 @default.
- W3033876676 cites W2755226689 @default.
- W3033876676 cites W2755264970 @default.
- W3033876676 cites W2755540120 @default.
- W3033876676 cites W2756452869 @default.
- W3033876676 cites W2766081121 @default.
- W3033876676 cites W2769410128 @default.
- W3033876676 cites W2788789092 @default.
- W3033876676 cites W2894675069 @default.
- W3033876676 cites W2943120662 @default.
- W3033876676 cites W2950457529 @default.
- W3033876676 cites W2993791299 @default.
- W3033876676 cites W4252755184 @default.
- W3033876676 cites W4293247451 @default.
- W3033876676 doi "https://doi.org/10.2174/1874285802014010113" @default.
- W3033876676 hasPublicationYear "2020" @default.
- W3033876676 type Work @default.
- W3033876676 sameAs 3033876676 @default.
- W3033876676 citedByCount "2" @default.
- W3033876676 countsByYear W30338766762020 @default.
- W3033876676 countsByYear W30338766762021 @default.
- W3033876676 crossrefType "journal-article" @default.
- W3033876676 hasAuthorship W3033876676A5001193473 @default.
- W3033876676 hasAuthorship W3033876676A5024850253 @default.
- W3033876676 hasAuthorship W3033876676A5065482785 @default.
- W3033876676 hasAuthorship W3033876676A5068143361 @default.
- W3033876676 hasAuthorship W3033876676A5090109619 @default.
- W3033876676 hasConcept C163994747 @default.
- W3033876676 hasConcept C181199279 @default.
- W3033876676 hasConcept C185592680 @default.
- W3033876676 hasConcept C2776527165 @default.
- W3033876676 hasConcept C2777261171 @default.
- W3033876676 hasConcept C2777272437 @default.
- W3033876676 hasConcept C43617362 @default.
- W3033876676 hasConcept C523546767 @default.
- W3033876676 hasConcept C54355233 @default.
- W3033876676 hasConcept C55493867 @default.
- W3033876676 hasConcept C86803240 @default.
- W3033876676 hasConceptScore W3033876676C163994747 @default.
- W3033876676 hasConceptScore W3033876676C181199279 @default.
- W3033876676 hasConceptScore W3033876676C185592680 @default.
- W3033876676 hasConceptScore W3033876676C2776527165 @default.
- W3033876676 hasConceptScore W3033876676C2777261171 @default.
- W3033876676 hasConceptScore W3033876676C2777272437 @default.
- W3033876676 hasConceptScore W3033876676C43617362 @default.
- W3033876676 hasConceptScore W3033876676C523546767 @default.
- W3033876676 hasConceptScore W3033876676C54355233 @default.
- W3033876676 hasConceptScore W3033876676C55493867 @default.
- W3033876676 hasConceptScore W3033876676C86803240 @default.
- W3033876676 hasIssue "1" @default.
- W3033876676 hasLocation W30338766761 @default.
- W3033876676 hasOpenAccess W3033876676 @default.
- W3033876676 hasPrimaryLocation W30338766761 @default.
- W3033876676 hasRelatedWork W2048472811 @default.
- W3033876676 hasRelatedWork W2053452659 @default.
- W3033876676 hasRelatedWork W2078768347 @default.
- W3033876676 hasRelatedWork W2136117239 @default.
- W3033876676 hasRelatedWork W2325988756 @default.
- W3033876676 hasRelatedWork W2348621492 @default.
- W3033876676 hasRelatedWork W2357773567 @default.
- W3033876676 hasRelatedWork W2404448771 @default.
- W3033876676 hasRelatedWork W2438210484 @default.
- W3033876676 hasRelatedWork W4234134618 @default.
- W3033876676 hasVolume "14" @default.
- W3033876676 isParatext "false" @default.
- W3033876676 isRetracted "false" @default.
- W3033876676 magId "3033876676" @default.