Matches in SemOpenAlex for { <https://semopenalex.org/work/W3033970087> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W3033970087 endingPage "1171" @default.
- W3033970087 startingPage "1166" @default.
- W3033970087 abstract "Purpose The main purpose of the current research work was to investigate the anticancer effects of Mahanimbine alkaloid in human bladder cancer cells along with examining its effects on cellular apoptosis, cell cycle phase distribution, and cell autophagy. Methods Cell viability was examined by WST-1 cell viability assay. Mahanimbine-induced apoptosis was examined by fluorescent microscopy using acridine orange (AO)/ethidium bromide (EB) staining as well as using flow cytometry in combination with annexin-v/propidium iodide (PI) staining. Further, western blot assay was used to study the effects of Mahanimbine on apoptosis-related protein expressions including Bax and Bcl-2. Autophagy induction was evaluated by transmission electron microscopy (TEM) and western blot. Flow cytometry was used to study the effects on cell cycle. Results The results showed that Mahanimbine decreased the viability of the human bladder cancer cells and exhibited an IC50 of 32.5 µM. The test molecule also caused remarkable changes in the morphology of human bladder cancer cells and inhibited their colony forming potential. The AO/EB staining assay showed that Mahanimbine inhibits the viability of cancer cells via induction of apoptotic cell death which was associated with increase in Bax and decrease in Bcl-2 levels. The apoptotic cells increased from 5.2% in control to around 75% at 100 µM concentration. Mahanimbine also led to dose-dependent G0/G1 cell cycle arrest. Autophagic vacuoles appeared in the treated cells indicating autophagic induction by the test molecule. The Mahanimbine-triggered autophagy was also linked with increase in the expression of LC3II and decrease in p62 expression. However, no apparent effects were observed on the LC3 I expression. Conclusion Taken together, the results of this study indicate that Mahanimbine natural product has the potential to be developed as a promising anticancer agent against human bladder carcinoma but further studies are needed to this direction." @default.
- W3033970087 created "2020-06-12" @default.
- W3033970087 creator A5035701638 @default.
- W3033970087 creator A5063329944 @default.
- W3033970087 creator A5066357292 @default.
- W3033970087 creator A5071183307 @default.
- W3033970087 creator A5082668462 @default.
- W3033970087 date "2020-03-01" @default.
- W3033970087 modified "2023-09-22" @default.
- W3033970087 title "Anticancer effects of Mahanimbine alkaloid on the human bladder cancer cells are due to the induction of G0/G1 cell cycle arrest, apoptosis and autophagy" @default.
- W3033970087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32521922" @default.
- W3033970087 hasPublicationYear "2020" @default.
- W3033970087 type Work @default.
- W3033970087 sameAs 3033970087 @default.
- W3033970087 citedByCount "3" @default.
- W3033970087 countsByYear W30339700872022 @default.
- W3033970087 countsByYear W30339700872023 @default.
- W3033970087 crossrefType "journal-article" @default.
- W3033970087 hasAuthorship W3033970087A5035701638 @default.
- W3033970087 hasAuthorship W3033970087A5063329944 @default.
- W3033970087 hasAuthorship W3033970087A5066357292 @default.
- W3033970087 hasAuthorship W3033970087A5071183307 @default.
- W3033970087 hasAuthorship W3033970087A5082668462 @default.
- W3033970087 hasConcept C105696609 @default.
- W3033970087 hasConcept C121608353 @default.
- W3033970087 hasConcept C153911025 @default.
- W3033970087 hasConcept C185592680 @default.
- W3033970087 hasConcept C190283241 @default.
- W3033970087 hasConcept C203522944 @default.
- W3033970087 hasConcept C2775934118 @default.
- W3033970087 hasConcept C2779102032 @default.
- W3033970087 hasConcept C2780783641 @default.
- W3033970087 hasConcept C29537977 @default.
- W3033970087 hasConcept C31573885 @default.
- W3033970087 hasConcept C53227056 @default.
- W3033970087 hasConcept C54355233 @default.
- W3033970087 hasConcept C553184892 @default.
- W3033970087 hasConcept C55493867 @default.
- W3033970087 hasConcept C86803240 @default.
- W3033970087 hasConcept C95444343 @default.
- W3033970087 hasConcept C96232424 @default.
- W3033970087 hasConceptScore W3033970087C105696609 @default.
- W3033970087 hasConceptScore W3033970087C121608353 @default.
- W3033970087 hasConceptScore W3033970087C153911025 @default.
- W3033970087 hasConceptScore W3033970087C185592680 @default.
- W3033970087 hasConceptScore W3033970087C190283241 @default.
- W3033970087 hasConceptScore W3033970087C203522944 @default.
- W3033970087 hasConceptScore W3033970087C2775934118 @default.
- W3033970087 hasConceptScore W3033970087C2779102032 @default.
- W3033970087 hasConceptScore W3033970087C2780783641 @default.
- W3033970087 hasConceptScore W3033970087C29537977 @default.
- W3033970087 hasConceptScore W3033970087C31573885 @default.
- W3033970087 hasConceptScore W3033970087C53227056 @default.
- W3033970087 hasConceptScore W3033970087C54355233 @default.
- W3033970087 hasConceptScore W3033970087C553184892 @default.
- W3033970087 hasConceptScore W3033970087C55493867 @default.
- W3033970087 hasConceptScore W3033970087C86803240 @default.
- W3033970087 hasConceptScore W3033970087C95444343 @default.
- W3033970087 hasConceptScore W3033970087C96232424 @default.
- W3033970087 hasIssue "2" @default.
- W3033970087 hasLocation W30339700871 @default.
- W3033970087 hasOpenAccess W3033970087 @default.
- W3033970087 hasPrimaryLocation W30339700871 @default.
- W3033970087 hasRelatedWork W1972110168 @default.
- W3033970087 hasRelatedWork W2176094125 @default.
- W3033970087 hasRelatedWork W2776951343 @default.
- W3033970087 hasRelatedWork W2789799276 @default.
- W3033970087 hasRelatedWork W2796466129 @default.
- W3033970087 hasRelatedWork W2802204039 @default.
- W3033970087 hasRelatedWork W2966939017 @default.
- W3033970087 hasRelatedWork W2980940781 @default.
- W3033970087 hasRelatedWork W2982117579 @default.
- W3033970087 hasRelatedWork W2989637184 @default.
- W3033970087 hasRelatedWork W2990275296 @default.
- W3033970087 hasRelatedWork W2990887413 @default.
- W3033970087 hasRelatedWork W2991442462 @default.
- W3033970087 hasRelatedWork W3016596592 @default.
- W3033970087 hasRelatedWork W3025665350 @default.
- W3033970087 hasRelatedWork W3026142979 @default.
- W3033970087 hasRelatedWork W3027416085 @default.
- W3033970087 hasRelatedWork W3081782977 @default.
- W3033970087 hasRelatedWork W3082065209 @default.
- W3033970087 hasRelatedWork W3107469800 @default.
- W3033970087 hasVolume "25" @default.
- W3033970087 isParatext "false" @default.
- W3033970087 isRetracted "false" @default.
- W3033970087 magId "3033970087" @default.
- W3033970087 workType "article" @default.