Matches in SemOpenAlex for { <https://semopenalex.org/work/W3033991735> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W3033991735 abstract "Chikungunya virus (CHIKV) is the causative agent of Aedes-borne tropical disease Chikungunya. Clinical manifestations of Chikungunya are highly similar with other Aedes-borne tropical diseases, often making diagnosis by physical examination alone inaccurate. Differential diagnosis of Chikungunya is done through serological or molecular testing, both of which are costly and tedious. Effort has been made by Agency for the Assessment and Application of Technology (BPPT) to construct a CHIKV diagnostic kit based on the interaction of IgM and IgG patient against the envelope proteins of the virus. Hence production of CHIKV envelope protein is essentially needed to develop the kit. This process could be done by expressing the CHIKV envelope protein E2 in the yeast Saccharomyces cerevisiae INVSc1. The aim of this research is to optimize the expression of recombinant CHIKV envelope protein E2 in the yeast. Incubation time is the subject to change in the yeast expression system, in which protein will be detected and compared using SDS-PAGE protocol. Recombinant clones in expression medium are incubated each for 0 h, 24 h, 48 h and 72 h. Proteins are most expressed after 72 hours incubation time. Further analysis with western blotting is needed to detect the expressed protein E2 CHIKV." @default.
- W3033991735 created "2020-06-12" @default.
- W3033991735 creator A5000140044 @default.
- W3033991735 creator A5022843360 @default.
- W3033991735 creator A5032938723 @default.
- W3033991735 creator A5062124961 @default.
- W3033991735 creator A5065609197 @default.
- W3033991735 date "2020-01-01" @default.
- W3033991735 modified "2023-10-16" @default.
- W3033991735 title "Incubation time optimization for the expression of recombinant CHIKV envelope protein E2 in the yeast Saccharomyces cerevisiae using SDS-PAGE" @default.
- W3033991735 cites W1899840761 @default.
- W3033991735 cites W2013947447 @default.
- W3033991735 cites W2028770473 @default.
- W3033991735 cites W2031822229 @default.
- W3033991735 cites W2051496342 @default.
- W3033991735 cites W2068988851 @default.
- W3033991735 cites W2088765906 @default.
- W3033991735 cites W2113728375 @default.
- W3033991735 cites W2138597477 @default.
- W3033991735 cites W2151156458 @default.
- W3033991735 cites W2467786533 @default.
- W3033991735 cites W2471213663 @default.
- W3033991735 cites W2559925268 @default.
- W3033991735 cites W2740184008 @default.
- W3033991735 cites W2911027880 @default.
- W3033991735 doi "https://doi.org/10.1063/5.0008985" @default.
- W3033991735 hasPublicationYear "2020" @default.
- W3033991735 type Work @default.
- W3033991735 sameAs 3033991735 @default.
- W3033991735 citedByCount "0" @default.
- W3033991735 crossrefType "proceedings-article" @default.
- W3033991735 hasAuthorship W3033991735A5000140044 @default.
- W3033991735 hasAuthorship W3033991735A5022843360 @default.
- W3033991735 hasAuthorship W3033991735A5032938723 @default.
- W3033991735 hasAuthorship W3033991735A5062124961 @default.
- W3033991735 hasAuthorship W3033991735A5065609197 @default.
- W3033991735 hasBestOaLocation W30339917351 @default.
- W3033991735 hasConcept C104317684 @default.
- W3033991735 hasConcept C143701427 @default.
- W3033991735 hasConcept C159047783 @default.
- W3033991735 hasConcept C2522874641 @default.
- W3033991735 hasConcept C25642318 @default.
- W3033991735 hasConcept C2777576037 @default.
- W3033991735 hasConcept C2779222958 @default.
- W3033991735 hasConcept C2780688631 @default.
- W3033991735 hasConcept C2781273456 @default.
- W3033991735 hasConcept C40767141 @default.
- W3033991735 hasConcept C533803919 @default.
- W3033991735 hasConcept C54355233 @default.
- W3033991735 hasConcept C55493867 @default.
- W3033991735 hasConcept C85265743 @default.
- W3033991735 hasConcept C86803240 @default.
- W3033991735 hasConceptScore W3033991735C104317684 @default.
- W3033991735 hasConceptScore W3033991735C143701427 @default.
- W3033991735 hasConceptScore W3033991735C159047783 @default.
- W3033991735 hasConceptScore W3033991735C2522874641 @default.
- W3033991735 hasConceptScore W3033991735C25642318 @default.
- W3033991735 hasConceptScore W3033991735C2777576037 @default.
- W3033991735 hasConceptScore W3033991735C2779222958 @default.
- W3033991735 hasConceptScore W3033991735C2780688631 @default.
- W3033991735 hasConceptScore W3033991735C2781273456 @default.
- W3033991735 hasConceptScore W3033991735C40767141 @default.
- W3033991735 hasConceptScore W3033991735C533803919 @default.
- W3033991735 hasConceptScore W3033991735C54355233 @default.
- W3033991735 hasConceptScore W3033991735C55493867 @default.
- W3033991735 hasConceptScore W3033991735C85265743 @default.
- W3033991735 hasConceptScore W3033991735C86803240 @default.
- W3033991735 hasLocation W30339917351 @default.
- W3033991735 hasOpenAccess W3033991735 @default.
- W3033991735 hasPrimaryLocation W30339917351 @default.
- W3033991735 hasRelatedWork W22818508 @default.
- W3033991735 hasRelatedWork W24170790 @default.
- W3033991735 hasRelatedWork W24548798 @default.
- W3033991735 hasRelatedWork W26430508 @default.
- W3033991735 hasRelatedWork W27749558 @default.
- W3033991735 hasRelatedWork W38331415 @default.
- W3033991735 hasRelatedWork W41315009 @default.
- W3033991735 hasRelatedWork W42082246 @default.
- W3033991735 hasRelatedWork W569784 @default.
- W3033991735 hasRelatedWork W6702747 @default.
- W3033991735 isParatext "false" @default.
- W3033991735 isRetracted "false" @default.
- W3033991735 magId "3033991735" @default.
- W3033991735 workType "article" @default.