FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3034125535> ?p ?o ?g. }

• W3034125535 endingPage "1379" @default.
• W3034125535 startingPage "1379" @default.
• W3034125535 abstract "Background: Endothelial cells from the microvasculature (hBMEC) of the brain show significant morphological and functional differences compared to EC from other anatomical areas. They are characterized by tight junctions, are not fenestrated and show less active transport mechanisms. On the other hand, the mitochondrial density is relatively high in hBMEC due to the high cerebral glucose metabolism. It could be already observed that interferon-α from SLE-sera induces the expression of MHC class I molecules on human dermal microendothelial cell line, but it is not known whether this also occurs on hBMEC. hBMECs can synthesize pro-inflammatory cytokines and chemokines such as IL-1β, but in lower concentrations than human umbilical vein endothelial cells. Patients suffering from systemic lupus erythematosus (SLE) or systemic sclerosis (SSc) show a wide spectrum of central nervous symptoms. Both, SLE and SSc, are characterised by different autoantibodies and endothelial vascular damage, especially in microvessels. 10-40% of patients with SLE suffer from lupus vasculopathy. Vascular dysfunction is one of the earliest pathological changes in SSc. Anti-endothelial autoantibodies (AECA) appear in SLE as well as in SSc and other connective tissue diseases. Research within the last years revealed that AECA play a critical role within the vascular pathogenesis of SLE and SSc. So far there is no evidence that AECA bind to hBMEC and it is not clear whether they have an effect on this special endothelial class. Objectives: In this project, we investigated if autoantibodies against hBMEC are detectable in SLE and SSc patients and if they have an influence on the activation of the endothelium by inducing adhesion molecules and on haemostasis by inducing factors of the clotting cascade. Methods: HiTrap Protein G HP antibody purification columns were used to purify IgG antibodies. Flow cytometry was used for analysis of autoantibodies against human cerebral microvascular endothelial cell line (hCMEC/D3). 26 sera of patients with SLE and 29 sera of patients with SSc were tested for presence of autoantibodies against hCMEC/D3. To analyse in vitro effects on hCMEC/D3, we measured changes in the expression of the following surface proteins: ICAM-1, VCAM-1, MHC class I and II, tissue factor, von-Willebrand-Factor, E-Selectin, P-Selectin, Thrombomodulin, CD73 and t-PA, each before and after three- and 24-hours incubation with IgG-fractions. IgG fractions of 12 SLE patients, 13 SSc patients and 13 healthy control persons (HC) were tested. Results: Autoantibodies against hCMEC/D3 were found in 21 of 26 patients with SLE (81%) and in 19 of 29 patients with SSc (66%) (p > 0.05) but not in healthy donors. After three hours incubation of hCMEC/D3 IgG-fractions, an upregulation of tissue factor by SSc-IgG (6.7% ± 5.2%) compared to HC-IgG (1.1% ± 2.8%, p < 0.01) and to SLE-IgG (1.6% ± 3.9%, p < 0.05), was detectable. There was no significant correlation between changes in surface protein expression and detection of ANA or of anti-hCMEC/D3 antibodies (p > 0.05). No change in expression of ICAM-1, VCAM-1, MHC class I and II, von-Willebrand-Factor, E-Selectin, P-Selectin, Thrombomodulin, CD73 and t-PA could be detected after incubation with IgG-fractions. Conclusion: Both, patients with SLE and patients with SSc showed autoantibodies against hBMEC. IgG fractions of patients with SSc, but not with SLE, induced an upregulation of tissue factor on the cell surface of hCMEC/D3. This could be an indicator for a direct pathogenic effect of AECA on hBMEC and might have an influence on haemostasis by activating the clotting cascade. Inhibition of these antibodies could reduce cerebral involvement of SSc. References: [1]Weksler BB, Subileau EA, Perriere N, et al. Blood-brain barrier-specific properties of a human adult brain endothelial cell line. Faseb J 2005;19:1872-1874. Disclosure of Interests: Rebecca Hasseli: None declared, Magdalena Maria Fürst: None declared, Pratibha Singh: None declared, Ulf Müller-Ladner Speakers bureau: Biogen, Manfred Kaps: None declared, Franz Blaes: None declared, Tibo Gerriets: None declared, Marlene Tschernatsch: None declared" @default.
• W3034125535 created "2020-06-12" @default.
• W3034125535 creator A5037302321 @default.
• W3034125535 creator A5038825414 @default.
• W3034125535 creator A5044124746 @default.
• W3034125535 creator A5048148281 @default.
• W3034125535 creator A5060330435 @default.
• W3034125535 creator A5066511500 @default.
• W3034125535 creator A5085784398 @default.
• W3034125535 creator A5088115834 @default.
• W3034125535 date "2020-06-01" @default.
• W3034125535 modified "2023-10-17" @default.
• W3034125535 title "AB0157 IGG FROM PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS AND SYSTEMIC SCLEROSIS HAVE AN INFLUENCE ON COAGULATION FACTORS IN HUMAN CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS IN-VITRO" @default.
• W3034125535 doi "https://doi.org/10.1136/annrheumdis-2020-eular.2631" @default.
• W3034125535 hasPublicationYear "2020" @default.
• W3034125535 type Work @default.
• W3034125535 sameAs 3034125535 @default.
• W3034125535 citedByCount "0" @default.
• W3034125535 crossrefType "journal-article" @default.
• W3034125535 hasAuthorship W3034125535A5037302321 @default.
• W3034125535 hasAuthorship W3034125535A5038825414 @default.
• W3034125535 hasAuthorship W3034125535A5044124746 @default.
• W3034125535 hasAuthorship W3034125535A5048148281 @default.
• W3034125535 hasAuthorship W3034125535A5060330435 @default.
• W3034125535 hasAuthorship W3034125535A5066511500 @default.
• W3034125535 hasAuthorship W3034125535A5085784398 @default.
• W3034125535 hasAuthorship W3034125535A5088115834 @default.
• W3034125535 hasConcept C123012128 @default.
• W3034125535 hasConcept C13373296 @default.
• W3034125535 hasConcept C134018914 @default.
• W3034125535 hasConcept C142724271 @default.
• W3034125535 hasConcept C159654299 @default.
• W3034125535 hasConcept C163764329 @default.
• W3034125535 hasConcept C202751555 @default.
• W3034125535 hasConcept C203014093 @default.
• W3034125535 hasConcept C2775915377 @default.
• W3034125535 hasConcept C2776670291 @default.
• W3034125535 hasConcept C2776912625 @default.
• W3034125535 hasConcept C2776914184 @default.
• W3034125535 hasConcept C2776992346 @default.
• W3034125535 hasConcept C2779075594 @default.
• W3034125535 hasConcept C2779134260 @default.
• W3034125535 hasConcept C2780942790 @default.
• W3034125535 hasConcept C2780972559 @default.
• W3034125535 hasConcept C55493867 @default.
• W3034125535 hasConcept C71924100 @default.
• W3034125535 hasConcept C86803240 @default.
• W3034125535 hasConceptScore W3034125535C123012128 @default.
• W3034125535 hasConceptScore W3034125535C13373296 @default.
• W3034125535 hasConceptScore W3034125535C134018914 @default.
• W3034125535 hasConceptScore W3034125535C142724271 @default.
• W3034125535 hasConceptScore W3034125535C159654299 @default.
• W3034125535 hasConceptScore W3034125535C163764329 @default.
• W3034125535 hasConceptScore W3034125535C202751555 @default.
• W3034125535 hasConceptScore W3034125535C203014093 @default.
• W3034125535 hasConceptScore W3034125535C2775915377 @default.
• W3034125535 hasConceptScore W3034125535C2776670291 @default.
• W3034125535 hasConceptScore W3034125535C2776912625 @default.
• W3034125535 hasConceptScore W3034125535C2776914184 @default.
• W3034125535 hasConceptScore W3034125535C2776992346 @default.
• W3034125535 hasConceptScore W3034125535C2779075594 @default.
• W3034125535 hasConceptScore W3034125535C2779134260 @default.
• W3034125535 hasConceptScore W3034125535C2780942790 @default.
• W3034125535 hasConceptScore W3034125535C2780972559 @default.
• W3034125535 hasConceptScore W3034125535C55493867 @default.
• W3034125535 hasConceptScore W3034125535C71924100 @default.
• W3034125535 hasConceptScore W3034125535C86803240 @default.
• W3034125535 hasIssue "Suppl 1" @default.
• W3034125535 hasLocation W30341255351 @default.
• W3034125535 hasOpenAccess W3034125535 @default.
• W3034125535 hasPrimaryLocation W30341255351 @default.
• W3034125535 hasRelatedWork W153140515 @default.
• W3034125535 hasRelatedWork W2027359080 @default.
• W3034125535 hasRelatedWork W2029625233 @default.
• W3034125535 hasRelatedWork W2177459785 @default.
• W3034125535 hasRelatedWork W2341928181 @default.
• W3034125535 hasRelatedWork W2387406902 @default.
• W3034125535 hasRelatedWork W2405627905 @default.
• W3034125535 hasRelatedWork W3143030268 @default.
• W3034125535 hasRelatedWork W4313386619 @default.
• W3034125535 hasRelatedWork W44765507 @default.
• W3034125535 hasVolume "79" @default.
• W3034125535 isParatext "false" @default.
• W3034125535 isRetracted "false" @default.
• W3034125535 magId "3034125535" @default.
• W3034125535 workType "article" @default.