FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3034150003> ?p ?o ?g. }

• W3034150003 endingPage "155146" @default.
• W3034150003 startingPage "155146" @default.
• W3034150003 abstract "Abstract Rheumatoid arthritis (RA) is a chronic, symmetric, systemic autoimmune disease. Because insufficient apoptosis of fibroblast-like synoviocytes (FLS) is an important characteristic of RA, promoting apoptosis is considered a potential therapeutic tool for treating RA. We have previously found that daphnetin (7,8-dihydroxycoumarin, DAP) has a pro-apoptotic effect on fibroblast-like synoviocytes from collagen-induced arthritis (CIA) rats. In the present study, we further investigated the mechanisms of DAP-induced apoptosis in CIA-FLS. CIA-FLS were incubated with DAP for 48 h in the presence or absence of caspase inhibitors, including inhibitors of caspase-3, caspase-8, or caspase-9 or a pan-caspase inhibitor; then, a series of experiments were performed to evaluate the mechanisms of DAP-induced apoptosis. Our results showed that DAP markedly decreased cell viability and induced the apoptosis of CIA-FLS along with typical morphological and ultrastructural changes; moreover, DAP increased FasL, cytochrome c (Cyt-c), Bax, caspase-3, caspase-8, and caspase-9 mRNA expression and Bax, caspase-3, caspase-8, and caspase-9 protein expression. In contrast, DAP decreased Bcl-2 mRNA and protein expression and promoted the release of Cyt-c from the mitochondria into the cytosol; these effects were attenuated to varying degrees by pre-treatment with caspase inhibitors, especially with caspase-3 or caspase-9 inhibitors or a pan-caspase inhibitor. In conclusion, the current findings demonstrate that the DAP-induced apoptosis of CIA-FLS occurred mainly via a caspase-dependent pathway, in particular the mitochondrial pathway, and that the Bax/Bcl-2 ratio was involved in this process. Thus, DAP may be a potential therapeutic agent for RA." @default.
• W3034150003 created "2020-06-12" @default.
• W3034150003 creator A5011747309 @default.
• W3034150003 creator A5022101283 @default.
• W3034150003 creator A5045539412 @default.
• W3034150003 creator A5062788280 @default.
• W3034150003 creator A5064441725 @default.
• W3034150003 creator A5068266670 @default.
• W3034150003 date "2020-09-01" @default.
• W3034150003 modified "2023-09-26" @default.
• W3034150003 title "Daphnetin induces apoptosis in fibroblast-like synoviocytes from collagen-induced arthritic rats mainly via the mitochondrial pathway" @default.
• W3034150003 cites W1530717069 @default.
• W3034150003 cites W1832285660 @default.
• W3034150003 cites W1998885858 @default.
• W3034150003 cites W2000468514 @default.
• W3034150003 cites W2003886322 @default.
• W3034150003 cites W2008757142 @default.
• W3034150003 cites W2009443499 @default.
• W3034150003 cites W2026884464 @default.
• W3034150003 cites W2028066934 @default.
• W3034150003 cites W2034230143 @default.
• W3034150003 cites W2041888407 @default.
• W3034150003 cites W2043814519 @default.
• W3034150003 cites W2045037598 @default.
• W3034150003 cites W2057356038 @default.
• W3034150003 cites W2062165600 @default.
• W3034150003 cites W2072760508 @default.
• W3034150003 cites W2085760547 @default.
• W3034150003 cites W2087906318 @default.
• W3034150003 cites W2088883749 @default.
• W3034150003 cites W2092708089 @default.
• W3034150003 cites W2110826367 @default.
• W3034150003 cites W2114570899 @default.
• W3034150003 cites W2136519986 @default.
• W3034150003 cites W2155993667 @default.
• W3034150003 cites W2258434529 @default.
• W3034150003 cites W2327398801 @default.
• W3034150003 cites W2416877399 @default.
• W3034150003 cites W2514782521 @default.
• W3034150003 cites W2920895255 @default.
• W3034150003 doi "https://doi.org/10.1016/j.cyto.2020.155146" @default.
• W3034150003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32505094" @default.
• W3034150003 hasPublicationYear "2020" @default.
• W3034150003 type Work @default.
• W3034150003 sameAs 3034150003 @default.
• W3034150003 citedByCount "4" @default.
• W3034150003 countsByYear W30341500032021 @default.
• W3034150003 countsByYear W30341500032022 @default.
• W3034150003 countsByYear W30341500032023 @default.
• W3034150003 crossrefType "journal-article" @default.
• W3034150003 hasAuthorship W3034150003A5011747309 @default.
• W3034150003 hasAuthorship W3034150003A5022101283 @default.
• W3034150003 hasAuthorship W3034150003A5045539412 @default.
• W3034150003 hasAuthorship W3034150003A5062788280 @default.
• W3034150003 hasAuthorship W3034150003A5064441725 @default.
• W3034150003 hasAuthorship W3034150003A5068266670 @default.
• W3034150003 hasConcept C185592680 @default.
• W3034150003 hasConcept C190283241 @default.
• W3034150003 hasConcept C202751555 @default.
• W3034150003 hasConcept C2780381497 @default.
• W3034150003 hasConcept C55493867 @default.
• W3034150003 hasConcept C86803240 @default.
• W3034150003 hasConcept C95444343 @default.
• W3034150003 hasConceptScore W3034150003C185592680 @default.
• W3034150003 hasConceptScore W3034150003C190283241 @default.
• W3034150003 hasConceptScore W3034150003C202751555 @default.
• W3034150003 hasConceptScore W3034150003C2780381497 @default.
• W3034150003 hasConceptScore W3034150003C55493867 @default.
• W3034150003 hasConceptScore W3034150003C86803240 @default.
• W3034150003 hasConceptScore W3034150003C95444343 @default.
• W3034150003 hasFunder F4320321001 @default.
• W3034150003 hasLocation W30341500031 @default.
• W3034150003 hasOpenAccess W3034150003 @default.
• W3034150003 hasPrimaryLocation W30341500031 @default.
• W3034150003 hasRelatedWork W1565632507 @default.
• W3034150003 hasRelatedWork W2348771880 @default.
• W3034150003 hasRelatedWork W2355359289 @default.
• W3034150003 hasRelatedWork W2355490605 @default.
• W3034150003 hasRelatedWork W2372432207 @default.
• W3034150003 hasRelatedWork W2375824747 @default.
• W3034150003 hasRelatedWork W2389099520 @default.
• W3034150003 hasRelatedWork W2805121851 @default.
• W3034150003 hasRelatedWork W3046665064 @default.
• W3034150003 hasRelatedWork W4220681557 @default.
• W3034150003 hasVolume "133" @default.
• W3034150003 isParatext "false" @default.
• W3034150003 isRetracted "false" @default.
• W3034150003 magId "3034150003" @default.
• W3034150003 workType "article" @default.