FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3034420007> ?p ?o ?g. }

• W3034420007 endingPage "50" @default.
• W3034420007 startingPage "40" @default.
• W3034420007 abstract "Introduction . The RHAMM (hyaluronan mediated mobility receptor) is overexpressed in many types of human cancer and increased synthesis of the RHAMM usually correlates with a poor prognostic factor. In this paper, we synthesized the peptide-RYQLHPYR modulating the activity of the RHAMM and examined the therapeutic potential of this RHAMM-targeting peptide as an antitumor agent. Objective . Study the effect of the synthetic peptide RYQLHPYR on viability, apoptosis, necrosis, caspase-3 / 7 activity, and invasion of prostate cancer cells. Materials and methods . The peptide RYQLHPYR was prepared by solid phase synthesis. Human prostate cancer cells (PC3 m-LN4), murine embryonic fibroblasts and murine embryonic fibroblasts (RHAMM- / -). To quantify the effect of the peptide on apoptosis and cell necrosis, ELISAPLUS was used. The activity of caspase-3 / 7 was determined by the colorimetric method. Evaluation of the anti-metastatic effect of the peptide in vitro was evaluated by invasion of cells by quantitative analysis of the area of degradation of fluorescent gelatin. Results . It was found that the peptide RYQLHPYR inhibited the growth of tumor cells PC3 m-LN4 at a concentration of 10 μg / ml (2 × 10–7 M) after 24 h by ~80 %. It was shown that the peptide stimulated the level of apoptosis in cancer cells, approximately 10-fold. It was found that the peptide increased the necrotic death of tumor cells by 2.5 times. During the research it was revealed that the peptide increased the caspase-3 / 7 activity in tumor cells by 2 times. At the same time, it was shown that RHAMM-targeting peptide had no significant effect on apoptosis and necrosis of normal cells (fibroblasts) and fibroblasts (RHAMM- / -). It was found that the peptide inhibited invasion of tumor cells by ~99.86 % at a concentration of 10 μg / ml (2 × 10–7 M). Conclusions . The obtained results indicate that the peptide RYQLHPYR has antitumor activity and, therefore, has a therapeutic potential for the treatment of prostate cancer." @default.
• W3034420007 created "2020-06-19" @default.
• W3034420007 creator A5019132179 @default.
• W3034420007 creator A5044063755 @default.
• W3034420007 date "2019-06-15" @default.
• W3034420007 modified "2023-10-01" @default.
• W3034420007 title "Study of antitumor activity of synthetic peptide ryqlhpyr on the prostate cancer cells" @default.
• W3034420007 cites W1544256999 @default.
• W3034420007 cites W1601051752 @default.
• W3034420007 cites W1757407923 @default.
• W3034420007 cites W1951950031 @default.
• W3034420007 cites W1970743383 @default.
• W3034420007 cites W1988750581 @default.
• W3034420007 cites W1989998611 @default.
• W3034420007 cites W2001547191 @default.
• W3034420007 cites W2002503549 @default.
• W3034420007 cites W2002507218 @default.
• W3034420007 cites W2002659318 @default.
• W3034420007 cites W2005393061 @default.
• W3034420007 cites W2010440856 @default.
• W3034420007 cites W2013602323 @default.
• W3034420007 cites W2022699678 @default.
• W3034420007 cites W2023028104 @default.
• W3034420007 cites W2027220201 @default.
• W3034420007 cites W2027471446 @default.
• W3034420007 cites W2035536358 @default.
• W3034420007 cites W2046308571 @default.
• W3034420007 cites W2047504386 @default.
• W3034420007 cites W2056217426 @default.
• W3034420007 cites W2058933525 @default.
• W3034420007 cites W2061354386 @default.
• W3034420007 cites W2080661253 @default.
• W3034420007 cites W2082815320 @default.
• W3034420007 cites W2083227014 @default.
• W3034420007 cites W2085527914 @default.
• W3034420007 cites W2086778699 @default.
• W3034420007 cites W2101148210 @default.
• W3034420007 cites W2103567156 @default.
• W3034420007 cites W2115903490 @default.
• W3034420007 cites W2117242540 @default.
• W3034420007 cites W2123735085 @default.
• W3034420007 cites W2126507337 @default.
• W3034420007 cites W2130988210 @default.
• W3034420007 cites W2137584261 @default.
• W3034420007 cites W2138509357 @default.
• W3034420007 cites W2141583129 @default.
• W3034420007 cites W2148692463 @default.
• W3034420007 cites W2157757076 @default.
• W3034420007 cites W2163491668 @default.
• W3034420007 cites W2171501507 @default.
• W3034420007 cites W2292424234 @default.
• W3034420007 cites W2435377468 @default.
• W3034420007 cites W2523333316 @default.
• W3034420007 cites W2589088938 @default.
• W3034420007 cites W2614966412 @default.
• W3034420007 cites W2768852352 @default.
• W3034420007 cites W2774407838 @default.
• W3034420007 cites W2804258023 @default.
• W3034420007 cites W2884753824 @default.
• W3034420007 cites W2891467397 @default.
• W3034420007 cites W2893157000 @default.
• W3034420007 cites W2902239815 @default.
• W3034420007 cites W2949950724 @default.
• W3034420007 cites W4211175230 @default.
• W3034420007 cites W49724973 @default.
• W3034420007 doi "https://doi.org/10.17650/1726-9784-2019-18-2-40-50" @default.
• W3034420007 hasPublicationYear "2019" @default.
• W3034420007 type Work @default.
• W3034420007 sameAs 3034420007 @default.
• W3034420007 citedByCount "1" @default.
• W3034420007 countsByYear W30344200072021 @default.
• W3034420007 crossrefType "journal-article" @default.
• W3034420007 hasAuthorship W3034420007A5019132179 @default.
• W3034420007 hasAuthorship W3034420007A5044063755 @default.
• W3034420007 hasBestOaLocation W30344200071 @default.
• W3034420007 hasConcept C104317684 @default.
• W3034420007 hasConcept C121608353 @default.
• W3034420007 hasConcept C126322002 @default.
• W3034420007 hasConcept C145103041 @default.
• W3034420007 hasConcept C153911025 @default.
• W3034420007 hasConcept C154317977 @default.
• W3034420007 hasConcept C17991360 @default.
• W3034420007 hasConcept C185592680 @default.
• W3034420007 hasConcept C190283241 @default.
• W3034420007 hasConcept C202751555 @default.
• W3034420007 hasConcept C203014093 @default.
• W3034420007 hasConcept C2779281246 @default.
• W3034420007 hasConcept C2780192828 @default.
• W3034420007 hasConcept C31573885 @default.
• W3034420007 hasConcept C502942594 @default.
• W3034420007 hasConcept C54355233 @default.
• W3034420007 hasConcept C55493867 @default.
• W3034420007 hasConcept C71924100 @default.
• W3034420007 hasConcept C81885089 @default.
• W3034420007 hasConcept C86803240 @default.
• W3034420007 hasConcept C96232424 @default.
• W3034420007 hasConceptScore W3034420007C104317684 @default.
• W3034420007 hasConceptScore W3034420007C121608353 @default.

• next