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• W3034733617 abstract "Gene silencing in bacteria is mediated by chromatin proteins, of which Escherichia coli H-NS is a paradigmatic example. H-NS forms multimeric nucleoprotein filaments with either one or two DNA duplexes, but the structures of these linear or bridged filaments remains unknown. Neither the determinants of H-NS genomic distribution nor the arrangements of H-NS DNA binding domains (DBDs) in linear or bridged filaments are currently defined. To address these questions, which are central to understanding gene silencing in bacteria, we developed a hybrid approach that combines a new method for in vitro chromatin reconstitution and mapping (Nitro-seq), ChIP-seq, nuclease-tagged DBDs to map DNA contacts, {middle dot}OH footprinting, molecular dynamics, and robust bioinformatic analyses. We find that DNA sequence principally governs genomic location of all filament types with indistinguishable sequence specificity, that DBD-DNA contacts vary in orientation and precise position with [~]10-bp spacing, and that unchanging contacts in linear and bridged filaments support a hemi-sequestration model of linear-to-bridged filament switching." @default.
• W3034733617 created "2020-06-19" @default.
• W3034733617 creator A5001862878 @default.
• W3034733617 creator A5010325555 @default.
• W3034733617 creator A5073073926 @default.
• W3034733617 creator A5073896140 @default.
• W3034733617 creator A5077227533 @default.
• W3034733617 creator A5087853985 @default.
• W3034733617 date "2020-06-12" @default.
• W3034733617 modified "2023-09-23" @default.
• W3034733617 title "Hybrid analysis reveals how DNA sequence governs genomic location and DNA contacts of bacterial chromatin H-NS filaments" @default.
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