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• W3034835114 abstract "Abstract A Trichinella spiralis aminopeptidase (TsAP) has been identified in intestinal infectious larvae (IIL) and adult worms (AW), but its biological function in the T. spiralis life cycle is unknown. The aim of this study was to characterize TsAP and ascertain its functions in the invasion, development and fecundity of T. spiralis . Recombinant TsAP (rTsAP) was expressed and purified. rTsAP has strong immunogenicity. qPCR and western blotting show that TsAP was transcribed and expressed at all T. spiralis lifecycle stages, but the expression level of TsAP mRNA and proteins at IIL and AW stages was obviously higher than those in muscle larvae (ML) and newborn larvae (NBL). The IFT results reveal that TsAP was principally located at the cuticle and the intrauterine embryos of this nematode. rTsAP had the enzymatic activity of natural aminopeptidase to hydrolyze the substrate Leu-pNA with an optimal temperature of 50 °C and optimal pH of 8.0. rTsAP promoted the larval penetration into intestinal epithelial cells, whereas anti-rTsAP antibodies suppressed the larval intrusion; the promotion and suppression was dose-dependently related to rTsAP or anti-rTsAP antibodies. TsAP protein expression level and enzymatic activity were reduced by 50.90 and 49.72% through silencing of the TsAP gene by specific siRNA 842. Intestinal AW and muscle larval burdens, worm length and female reproductive capacity were significantly declined in mice infected with siRNA-transfected ML compared to the control siRNA and PBS group. These results indicate that TsAP participates in the invasion, development and fecundity of T. spiralis and it might be a candidate target for anti- Trichinella vaccines." @default.
• W3034835114 created "2020-06-19" @default.
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• W3034835114 date "2020-06-15" @default.
• W3034835114 modified "2023-10-18" @default.
• W3034835114 title "Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity" @default.
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• W3034835114 doi "https://doi.org/10.1186/s13567-020-00805-w" @default.
• W3034835114 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7296678" @default.
• W3034835114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32539772" @default.
• W3034835114 hasPublicationYear "2020" @default.
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