Matches in SemOpenAlex for { <https://semopenalex.org/work/W3035011721> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3035011721 abstract "Exosomes, nanosized extracellular vesicles, may be key to translating multipotent stromal cell (MSC) therapy to the bedside. We previously found that nuclear factor erythroid 2-related factor 2 (Nrf2) regulates MSC promotion of tissue repair in diabetes. Here, we explore a novel role of Nrf2 in exosome biogenesis and investigate whether exosome treatment recapitulates the effects MSCs have on wound healing in mice with type 2 diabetes. Exosomes were harvested by differential ultracentrifugation of conditioned human bone marrow derived MSC media. For Nrf2-active exosomes, MSCs were incubated with potent Nrf2 activator, CDDO-Im. MSC characterization demonstrated robust tri-lineage differentiation, >95% expression of positive markers (CD44/CD73/CD90/CD105/CD106), and <5% expression of negative markers (CD45/CD31/CD14/CD19/HLA-DR). Immunoblotting of exosome isolates demonstrates enrichment for CD81, CD9 and TSG101. Nanoparticle tracking analysis demonstrates that Nrf2-active MSCs increase exosome secretion by 54%, compared to Nrf2-baseline MSCs (p<0.05). Exosomes were injected into the wound margin of full-thickness wounds on Leprdb/db (db/db) mice. Both Nrf2-baseline and Nrf2-active exosome treatment significantly reduced closure time to 15.5 and 14 days respectively, compared to 29.8 days for vehicle-treated wounds (p<0.05). This reduction eliminated the delay in closure time compared to wounds of C57/B6 mice. Nrf2-active exosome treatment of db/db wounds reduced closure time by a further 2.6 days compared to untreated C57/B6 wounds. At day 10, exosome treated db/db wounds have significant decreases in epithelial gap, expanded granulation tissue, and greater density of CD31+ vessels compared to vehicle-treated wounds. We conclude that activating Nrf2 in MSCs increases isolation efficiency by amplifying exosome yield and content. MSC exosome based therapies have potential for rapid translation to improve clinical wound healing outcomes. Disclosure J. Kuhn: None. A. Hassan: None. S. Sharma: None. J. Kwong: None. M. Rahman: None. S. Adam: None. J. Lee: None. A.P. Villarreal Ponce: None. P.S. Rabbani: None. Funding Plastic Surgery Foundation; Wound Healing Society; New York University Clinical and Translational Science Institute" @default.
- W3035011721 created "2020-06-19" @default.
- W3035011721 creator A5012139007 @default.
- W3035011721 creator A5038234918 @default.
- W3035011721 creator A5040025655 @default.
- W3035011721 creator A5042975086 @default.
- W3035011721 creator A5050461210 @default.
- W3035011721 creator A5057297868 @default.
- W3035011721 creator A5062553698 @default.
- W3035011721 creator A5070017206 @default.
- W3035011721 creator A5080236938 @default.
- W3035011721 date "2020-06-01" @default.
- W3035011721 modified "2023-10-17" @default.
- W3035011721 title "594-P: Human Nrf2-Active Multipotent Stromal Cell Exosomes Promote Tissue Repair in a Wound Model of Type 2 Diabetes" @default.
- W3035011721 doi "https://doi.org/10.2337/db20-594-p" @default.
- W3035011721 hasPublicationYear "2020" @default.
- W3035011721 type Work @default.
- W3035011721 sameAs 3035011721 @default.
- W3035011721 citedByCount "0" @default.
- W3035011721 crossrefType "journal-article" @default.
- W3035011721 hasAuthorship W3035011721A5012139007 @default.
- W3035011721 hasAuthorship W3035011721A5038234918 @default.
- W3035011721 hasAuthorship W3035011721A5040025655 @default.
- W3035011721 hasAuthorship W3035011721A5042975086 @default.
- W3035011721 hasAuthorship W3035011721A5050461210 @default.
- W3035011721 hasAuthorship W3035011721A5057297868 @default.
- W3035011721 hasAuthorship W3035011721A5062553698 @default.
- W3035011721 hasAuthorship W3035011721A5070017206 @default.
- W3035011721 hasAuthorship W3035011721A5080236938 @default.
- W3035011721 hasConcept C104317684 @default.
- W3035011721 hasConcept C116716535 @default.
- W3035011721 hasConcept C145059251 @default.
- W3035011721 hasConcept C1491633281 @default.
- W3035011721 hasConcept C16685009 @default.
- W3035011721 hasConcept C16930146 @default.
- W3035011721 hasConcept C185592680 @default.
- W3035011721 hasConcept C198826908 @default.
- W3035011721 hasConcept C203014093 @default.
- W3035011721 hasConcept C20518536 @default.
- W3035011721 hasConcept C2780269544 @default.
- W3035011721 hasConcept C2780932548 @default.
- W3035011721 hasConcept C2781261824 @default.
- W3035011721 hasConcept C2908689518 @default.
- W3035011721 hasConcept C502942594 @default.
- W3035011721 hasConcept C55493867 @default.
- W3035011721 hasConcept C71924100 @default.
- W3035011721 hasConcept C86803240 @default.
- W3035011721 hasConcept C95444343 @default.
- W3035011721 hasConceptScore W3035011721C104317684 @default.
- W3035011721 hasConceptScore W3035011721C116716535 @default.
- W3035011721 hasConceptScore W3035011721C145059251 @default.
- W3035011721 hasConceptScore W3035011721C1491633281 @default.
- W3035011721 hasConceptScore W3035011721C16685009 @default.
- W3035011721 hasConceptScore W3035011721C16930146 @default.
- W3035011721 hasConceptScore W3035011721C185592680 @default.
- W3035011721 hasConceptScore W3035011721C198826908 @default.
- W3035011721 hasConceptScore W3035011721C203014093 @default.
- W3035011721 hasConceptScore W3035011721C20518536 @default.
- W3035011721 hasConceptScore W3035011721C2780269544 @default.
- W3035011721 hasConceptScore W3035011721C2780932548 @default.
- W3035011721 hasConceptScore W3035011721C2781261824 @default.
- W3035011721 hasConceptScore W3035011721C2908689518 @default.
- W3035011721 hasConceptScore W3035011721C502942594 @default.
- W3035011721 hasConceptScore W3035011721C55493867 @default.
- W3035011721 hasConceptScore W3035011721C71924100 @default.
- W3035011721 hasConceptScore W3035011721C86803240 @default.
- W3035011721 hasConceptScore W3035011721C95444343 @default.
- W3035011721 hasIssue "Supplement_1" @default.
- W3035011721 hasLocation W30350117211 @default.
- W3035011721 hasOpenAccess W3035011721 @default.
- W3035011721 hasPrimaryLocation W30350117211 @default.
- W3035011721 hasRelatedWork W2510844606 @default.
- W3035011721 hasRelatedWork W2785527513 @default.
- W3035011721 hasRelatedWork W2955875101 @default.
- W3035011721 hasRelatedWork W2972642636 @default.
- W3035011721 hasRelatedWork W3087725815 @default.
- W3035011721 hasRelatedWork W3139087999 @default.
- W3035011721 hasRelatedWork W3198772284 @default.
- W3035011721 hasRelatedWork W3214237393 @default.
- W3035011721 hasRelatedWork W4200367686 @default.
- W3035011721 hasRelatedWork W4386274136 @default.
- W3035011721 hasVolume "69" @default.
- W3035011721 isParatext "false" @default.
- W3035011721 isRetracted "false" @default.
- W3035011721 magId "3035011721" @default.
- W3035011721 workType "article" @default.