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• W3035498864 abstract "Abstract The aim of the study was to describe the metabolic responses of protein metabolism to a period of negative nutrient balance induced by feed restriction (FR). Seven multiparous Holstein cows (93 ± 15 days in milk) were randomly assigned to 7 treatments in a 7 × 4 Youden square design. Daily intake was restricted to provide 60% of energy requirements during 5 d except for one treatment with ad libitum (AL) feeding. While 5 out of 7 experimental treatments involved abomasal supplementation of AA or glucose, in this article we evaluated only the effects of FR by comparing both control treatments (AL and FR). Data of 2 cows within the AL group were removed due to sickness and therefore it had n = 2. A rapid decrease of most amino acids in plasma was paired with an increase in blood urea N with its peak on d 2 and decreasing afterwards. On the other hand, Lys, Arg, Gly, Gln, and Cys were greater during FR. Comparing the fluctuation of all the essayed N components in circulation across the 5-d period, protein tissue mobilization may have supplied amino acids for catabolism to provide needs for N and energy precursors. Implications The short-term feed restriction model described in this article can serve as an alternative to study metabolic adaptations during the transition period. The response observed of the protein metabolism sets the baseline to measure the effect of nutrients supplementation and identify those candidates that will improve milk production and overall health after calving." @default.
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• W3035498864 date "2020-06-13" @default.
• W3035498864 modified "2023-09-27" @default.
• W3035498864 title "Feed restriction in mid-lactation dairy cows. II: Effects on protein metabolism-related blood metabolites" @default.
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• W3035498864 doi "https://doi.org/10.1101/2020.06.12.144980" @default.
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