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• W3035505511 abstract "Abstract T lymphocytes are key components in adaptive immunity and their activation naturally involves mitochondrial-derived oxygen species (mtROS). In particular, H 2 O 2 has been implicated as an important signaling molecule regulating major T cell functions. H 2 O 2 targets the oxidation status of functional cysteine residues but knowledge if and where this happens in T cell signaling networks is widely missing. This study aimed to identify mtROS-sensitive processes in activated primary human CD4 + T cells. By using a thiol-specific redox proteomic approach we examined the oxidation state of 4784 cysteine-containing peptides of ex vivo stimulated T cells from healthy individuals. Upon activation, a shift in oxidation was observed at catalytic cysteine residues of peroxiredoxins (PRDX5 & PRDX6), and T cells were found to maintain their global thiol-redox homeostasis. In parallel, a distinct set of 88 cysteine residues were found to be differentially oxidized upon T cell activation suggesting novel functional thiol switches. In mitochondria, cysteine oxidations selectively modified regulators of respiration (NDUFA2, NDUFA8, and UQCRH) confirming electron leakage from electron transport complexes I and III. The majority of oxidations occurred outside mitochondria and enriched sensitive thiols at regulators of cytoskeleton dynamics (e.g. CYFIP2 and ARPC1B) and known immune functions including the non-receptor tyrosine phosphatase PTPN7. Conversely, cysteine reduction occurred predominantly at transcriptional regulators and sites that coordinate zinc-binding in zinc-finger motifs. Indeed, fluorescence microscopy revealed a colocalization of zinc-rich microenvironments and mitochondria in T cells suggesting mtROS-dependent zinc-release of identified transcriptional regulators including ZFP36, RPL37A and CRIP2. In conclusion, this study complements knowledge on the mtROS signaling network and suggests zinc-dependent thiol switches as a mechanism of how mtROS affects transcription and translation in T cells." @default.
• W3035505511 created "2020-06-19" @default.
• W3035505511 creator A5042906158 @default.
• W3035505511 creator A5047962558 @default.
• W3035505511 creator A5050930433 @default.
• W3035505511 creator A5067537415 @default.
• W3035505511 creator A5080890730 @default.
• W3035505511 creator A5088547780 @default.
• W3035505511 date "2020-06-15" @default.
• W3035505511 modified "2023-10-16" @default.
• W3035505511 title "Identification of mtROS-sensitive processes in activated CD4+T cells" @default.
• W3035505511 cites W1495595397 @default.
• W3035505511 cites W1510592624 @default.
• W3035505511 cites W1531010890 @default.
• W3035505511 cites W1587246273 @default.
• W3035505511 cites W1587695215 @default.
• W3035505511 cites W1610775872 @default.
• W3035505511 cites W1948683976 @default.
• W3035505511 cites W1963902368 @default.
• W3035505511 cites W1965879808 @default.
• W3035505511 cites W1966485211 @default.
• W3035505511 cites W1967727543 @default.
• W3035505511 cites W1969665448 @default.
• W3035505511 cites W1974966731 @default.
• W3035505511 cites W1981593008 @default.
• W3035505511 cites W1982753455 @default.
• W3035505511 cites W1983016535 @default.
• W3035505511 cites W1983078193 @default.
• W3035505511 cites W1983491805 @default.
• W3035505511 cites W1984136589 @default.
• W3035505511 cites W1986304086 @default.
• W3035505511 cites W1987632882 @default.
• W3035505511 cites W1991933429 @default.
• W3035505511 cites W1993723357 @default.
• W3035505511 cites W2000523097 @default.
• W3035505511 cites W2002611021 @default.
• W3035505511 cites W2005478135 @default.
• W3035505511 cites W2009808081 @default.
• W3035505511 cites W2010478212 @default.
• W3035505511 cites W2013422007 @default.
• W3035505511 cites W2014389088 @default.
• W3035505511 cites W2015650234 @default.
• W3035505511 cites W2019243604 @default.
• W3035505511 cites W2023436768 @default.
• W3035505511 cites W2025236053 @default.
• W3035505511 cites W2027756065 @default.
• W3035505511 cites W2028321471 @default.
• W3035505511 cites W2030802179 @default.
• W3035505511 cites W2035127779 @default.
• W3035505511 cites W2037525195 @default.
• W3035505511 cites W2038714516 @default.
• W3035505511 cites W2040529452 @default.
• W3035505511 cites W2041687431 @default.
• W3035505511 cites W2042581720 @default.
• W3035505511 cites W2043096460 @default.
• W3035505511 cites W2051861425 @default.
• W3035505511 cites W2054236535 @default.
• W3035505511 cites W2054633904 @default.
• W3035505511 cites W2055330285 @default.
• W3035505511 cites W2065306454 @default.
• W3035505511 cites W2070807787 @default.
• W3035505511 cites W2072659663 @default.
• W3035505511 cites W2076321627 @default.
• W3035505511 cites W2079999658 @default.
• W3035505511 cites W2083052219 @default.
• W3035505511 cites W2083539187 @default.
• W3035505511 cites W2083595146 @default.
• W3035505511 cites W2085069618 @default.
• W3035505511 cites W2089818796 @default.
• W3035505511 cites W2093788956 @default.
• W3035505511 cites W2098425296 @default.
• W3035505511 cites W2098504210 @default.
• W3035505511 cites W2106179675 @default.
• W3035505511 cites W2106570152 @default.
• W3035505511 cites W2110271242 @default.
• W3035505511 cites W2112025608 @default.
• W3035505511 cites W2118354969 @default.
• W3035505511 cites W2121103215 @default.
• W3035505511 cites W2122271393 @default.
• W3035505511 cites W2122341467 @default.
• W3035505511 cites W2134958153 @default.
• W3035505511 cites W2139873559 @default.
• W3035505511 cites W2140014506 @default.
• W3035505511 cites W2141416399 @default.
• W3035505511 cites W2142261476 @default.
• W3035505511 cites W2143010594 @default.
• W3035505511 cites W2155355681 @default.
• W3035505511 cites W2157485133 @default.
• W3035505511 cites W2157579772 @default.
• W3035505511 cites W2159410139 @default.
• W3035505511 cites W2160472691 @default.
• W3035505511 cites W2160606731 @default.
• W3035505511 cites W2164156889 @default.
• W3035505511 cites W2164683872 @default.
• W3035505511 cites W2167029847 @default.
• W3035505511 cites W2170812873 @default.
• W3035505511 cites W2171879063 @default.
• W3035505511 cites W2173980345 @default.
• W3035505511 cites W2242959768 @default.
• W3035505511 cites W2304223963 @default.

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