FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3035598905> ?p ?o ?g. }

• W3035598905 endingPage "2846" @default.
• W3035598905 startingPage "2839" @default.
• W3035598905 abstract "A novel coronavirus associated with acute respiratory disease (named SARS-CoV-2) is recently identified in Wuhan city, China, spread rapidly worldwide. Early identification of this novel coronavirus by molecular tools is critical for surveillance and control of the epidemic outbreak. We aimed to establish a simple method for the detection of SARS-CoV-2 in differentiating with SARS-CoV. Primers of our in-house reverse transcription polymerase chain reaction (RT-PCR) assays were designed to target conserved regions of the RdRP gene and E gene, selected restriction enzymes EcoRI, Tsp45I, and AluI to distinguish between SARS-CoV-2 and SARS-CoV. In this report, a 396-bp fragment of the RdRp gene and 345-bp fragment of the E gene were amplified by one-step RT-PCR. Enzyme Tsp45I cuts the RdRP-amplified product of SARS-CoV-2 generating three fragments of 45, 154, and 197 bp, but it did not cut the amplicon of SARS-CoV. In contrast, the amplified product of SARS-CoV was digested with EcoRI producing two fragments of 76 and 320 bp, whereas the amplicon of SARS-CoV-2 was undigested by Tsp45I help to distinguish clearly SARS-CoV-2 from SARS-CoV on gel electrophoresis. In addition, AluI cut the amplicon of the E gene of SARS-CoV-2 generating two fragments of 248 and 97 bp without cutting to SARS-CoV. The accuracy of the assay was confirmed by sequencing and phylogenetic analysis. When evaluated on clinical samples showed a high sensitivity of 95%, specificity of our assay was 100% and clinical performance for detection of SARS-CoV-2 in comparison with other reference assays. In conclusion, in the present study, we successfully developed a simple method for molecular detection of SARS-CoV-2 in differentiating with SARS-CoV." @default.
• W3035598905 created "2020-06-19" @default.
• W3035598905 creator A5027939526 @default.
• W3035598905 creator A5029206903 @default.
• W3035598905 creator A5030418904 @default.
• W3035598905 creator A5039815800 @default.
• W3035598905 creator A5041532071 @default.
• W3035598905 creator A5042162157 @default.
• W3035598905 creator A5043268016 @default.
• W3035598905 creator A5048478579 @default.
• W3035598905 creator A5049629850 @default.
• W3035598905 creator A5049663752 @default.
• W3035598905 creator A5054528135 @default.
• W3035598905 creator A5056525892 @default.
• W3035598905 creator A5063970221 @default.
• W3035598905 creator A5065087125 @default.
• W3035598905 creator A5071642658 @default.
• W3035598905 creator A5089720287 @default.
• W3035598905 date "2020-06-29" @default.
• W3035598905 modified "2023-10-12" @default.
• W3035598905 title "A simple method for detection of a novel coronavirus (SARS‐CoV‐2) using one‐step RT‐PCR followed by restriction fragment length polymorphism" @default.
• W3035598905 cites W2884280018 @default.
• W3035598905 cites W3001195213 @default.
• W3035598905 cites W3001456238 @default.
• W3035598905 cites W3001897055 @default.
• W3035598905 cites W3002539152 @default.
• W3035598905 cites W3002812395 @default.
• W3035598905 cites W3003637715 @default.
• W3035598905 cites W3003951199 @default.
• W3035598905 cites W3004318991 @default.
• W3035598905 cites W3005510968 @default.
• W3035598905 cites W3005802419 @default.
• W3035598905 cites W3008627212 @default.
• W3035598905 cites W3021555206 @default.
• W3035598905 cites W3035598905 @default.
• W3035598905 cites W4206023641 @default.
• W3035598905 cites W4206822952 @default.
• W3035598905 cites W4206833075 @default.
• W3035598905 doi "https://doi.org/10.1002/jmv.26171" @default.
• W3035598905 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7307074" @default.
• W3035598905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32530490" @default.
• W3035598905 hasPublicationYear "2020" @default.
• W3035598905 type Work @default.
• W3035598905 sameAs 3035598905 @default.
• W3035598905 citedByCount "7" @default.
• W3035598905 countsByYear W30355989052020 @default.
• W3035598905 countsByYear W30355989052021 @default.
• W3035598905 countsByYear W30355989052022 @default.
• W3035598905 crossrefType "journal-article" @default.
• W3035598905 hasAuthorship W3035598905A5027939526 @default.
• W3035598905 hasAuthorship W3035598905A5029206903 @default.
• W3035598905 hasAuthorship W3035598905A5030418904 @default.
• W3035598905 hasAuthorship W3035598905A5039815800 @default.
• W3035598905 hasAuthorship W3035598905A5041532071 @default.
• W3035598905 hasAuthorship W3035598905A5042162157 @default.
• W3035598905 hasAuthorship W3035598905A5043268016 @default.
• W3035598905 hasAuthorship W3035598905A5048478579 @default.
• W3035598905 hasAuthorship W3035598905A5049629850 @default.
• W3035598905 hasAuthorship W3035598905A5049663752 @default.
• W3035598905 hasAuthorship W3035598905A5054528135 @default.
• W3035598905 hasAuthorship W3035598905A5056525892 @default.
• W3035598905 hasAuthorship W3035598905A5063970221 @default.
• W3035598905 hasAuthorship W3035598905A5065087125 @default.
• W3035598905 hasAuthorship W3035598905A5071642658 @default.
• W3035598905 hasAuthorship W3035598905A5089720287 @default.
• W3035598905 hasBestOaLocation W30355989051 @default.
• W3035598905 hasConcept C104317684 @default.
• W3035598905 hasConcept C128319531 @default.
• W3035598905 hasConcept C130123407 @default.
• W3035598905 hasConcept C142724271 @default.
• W3035598905 hasConcept C153911025 @default.
• W3035598905 hasConcept C159047783 @default.
• W3035598905 hasConcept C168007829 @default.
• W3035598905 hasConcept C2777648638 @default.
• W3035598905 hasConcept C2779134260 @default.
• W3035598905 hasConcept C3008058167 @default.
• W3035598905 hasConcept C49105822 @default.
• W3035598905 hasConcept C524204448 @default.
• W3035598905 hasConcept C54355233 @default.
• W3035598905 hasConcept C71924100 @default.
• W3035598905 hasConcept C8185291 @default.
• W3035598905 hasConcept C84289048 @default.
• W3035598905 hasConcept C86803240 @default.
• W3035598905 hasConceptScore W3035598905C104317684 @default.
• W3035598905 hasConceptScore W3035598905C128319531 @default.
• W3035598905 hasConceptScore W3035598905C130123407 @default.
• W3035598905 hasConceptScore W3035598905C142724271 @default.
• W3035598905 hasConceptScore W3035598905C153911025 @default.
• W3035598905 hasConceptScore W3035598905C159047783 @default.
• W3035598905 hasConceptScore W3035598905C168007829 @default.
• W3035598905 hasConceptScore W3035598905C2777648638 @default.
• W3035598905 hasConceptScore W3035598905C2779134260 @default.
• W3035598905 hasConceptScore W3035598905C3008058167 @default.
• W3035598905 hasConceptScore W3035598905C49105822 @default.
• W3035598905 hasConceptScore W3035598905C524204448 @default.
• W3035598905 hasConceptScore W3035598905C54355233 @default.
• W3035598905 hasConceptScore W3035598905C71924100 @default.
• W3035598905 hasConceptScore W3035598905C8185291 @default.

• next