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- W3035777701 abstract "Developments in fluorescence in situ hybridization accrued over recent years now make it possible to detect and precisely localize nucleic acid sequences with a sensitivity sufficient to detect single copy genes or their primary transcripts (Reviewed in Ref.1). These advances have essentially revolutionized the field of molecular cytogenetics, providing not only a powerful approach to gene mapping and genetic diagnosis, but a new way to probe the higher level organization of the nucleus and chromosome. The methodology we and others primarily utilize is based on non-isotopic labelling of DNA probes with moieties such as biotin or digoxygenin, which are detected by fluorochrome-conjugated avidin or anti-digoxygenin antibodies. Alternatively, probes directly labeled with fluorochromes have recently become available. Appropriate modifications of protocols allow either cellular DNA or RNA to be targeted, and hybridization can be to intact cells, nuclei, or chromosomes." @default.
- W3035777701 created "2020-06-25" @default.
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- W3035777701 date "1992-08-01" @default.
- W3035777701 modified "2023-09-27" @default.
- W3035777701 title "Fluorescence in situ hybridization: Investigating the functional organization of genes and RNAs" @default.
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- W3035777701 doi "https://doi.org/10.1017/s0424820100123192" @default.
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