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- W3035784095 abstract "As a series of histochemical studies on the mucosubstances of the granulocytes in this laboratory, the results obtained by electron microscopic radioautography and X-ray microanalysis have been reported. The femur bone marrows of adult rabbits weighing 1. 0-1. 5kg were diced into small pieces and incubated for 10, 30 and 60 minutes in Eagle's MEM supplemented with 10 % bovine serum, containing radioactive carrier free sulfuric acid at a concentration of lmCi/ml. They were fixed doubly in glutaraldehyde buffered with 0. 1 M phosphate buffer (pH 7. 2) and osmium tetroxide buffered with 0. 1 M phosphate buffer(pH 7. 2), dehydrated through a graded series of ethanol and acetone and embedded in Epon. Ultrathin sections were cut with an LKB ultrotome and radioautographed with Sakura NR-H2 emulsion by wire loop method, exposed for 127-203 days, developed with phenidone developer after gold latensification, stained with lead citrate and observed in Hitachi HS-9 electron microscope." @default.
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- W3035784095 date "1978-08-01" @default.
- W3035784095 modified "2023-09-27" @default.
- W3035784095 title "Mucosubstances of the granulocytes studied by means of electron microscopic radioautography and X-ray microanalysis" @default.
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- W3035784095 doi "https://doi.org/10.1017/s0424820100083138" @default.
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