Matches in SemOpenAlex for { <https://semopenalex.org/work/W3035825831> ?p ?o ?g. }
- W3035825831 abstract "Abstract Keratin intermediate filaments (IFs) convey mechanical stability and protection against stress to epithelial cells, and may participate in nuclear structure and organization. Keratins are important for colon health as observed in keratin 8 knockout (K8 −/− ) mice, which exhibit colonic inflammation and epithelial hyperproliferation. Here, using a full body and two intestinal epithelial-specific K8 −/− knockout mouse models, we determine if cytoplasmic keratins affect the nuclear structure and lamina in epithelial colonocytes. K8 −/− colonocytes in vivo and in organoid cultures exhibit significantly decreased levels of the major lamins A/C, B1 and B2 in a colon-specific and cell-intrinsic manner independent of major changes in colonic inflammation or microbiota. Downregulation of K8 by siRNA in Caco-2 cells similarly decreases lamin A levels, which recover after re-expression of K8. K8 loss is associated with reduced plectin, LINC complex proteins and lamin-associated proteins, indicating a dysfunctional keratin-nuclear lamina coupling. Immunoprecipitation identifies complexes of colonocyte keratins with the LINC protein SUN2 and lamin A. Hyperphosphorylation of the lamin A-associated cell cycle regulator pRb in K8 −/− colonocytes together with increased nuclear localization of the mechanosensor YAP provide a molecular mechanism for the hyperproliferation phenotype. These findings identify a novel, colonocyte-specific role for K8 in nuclear function." @default.
- W3035825831 created "2020-06-25" @default.
- W3035825831 creator A5004905921 @default.
- W3035825831 creator A5008739379 @default.
- W3035825831 creator A5020529360 @default.
- W3035825831 creator A5030423855 @default.
- W3035825831 creator A5036408818 @default.
- W3035825831 creator A5049643687 @default.
- W3035825831 creator A5050356845 @default.
- W3035825831 creator A5066897632 @default.
- W3035825831 date "2020-06-22" @default.
- W3035825831 modified "2023-09-27" @default.
- W3035825831 title "Keratins couple with the nuclear lamina and regulate proliferation in colonic epithelial cells" @default.
- W3035825831 cites W1514903902 @default.
- W3035825831 cites W1526450413 @default.
- W3035825831 cites W1536843121 @default.
- W3035825831 cites W1541500240 @default.
- W3035825831 cites W1857005108 @default.
- W3035825831 cites W1881314992 @default.
- W3035825831 cites W1967964558 @default.
- W3035825831 cites W1995160678 @default.
- W3035825831 cites W1999330214 @default.
- W3035825831 cites W2017830084 @default.
- W3035825831 cites W2022156993 @default.
- W3035825831 cites W2023738576 @default.
- W3035825831 cites W2027150010 @default.
- W3035825831 cites W2029179126 @default.
- W3035825831 cites W2034962229 @default.
- W3035825831 cites W2041599232 @default.
- W3035825831 cites W2052386537 @default.
- W3035825831 cites W2057628471 @default.
- W3035825831 cites W2068343781 @default.
- W3035825831 cites W2071597617 @default.
- W3035825831 cites W2071624086 @default.
- W3035825831 cites W2072320390 @default.
- W3035825831 cites W2075658584 @default.
- W3035825831 cites W2075817848 @default.
- W3035825831 cites W2096539266 @default.
- W3035825831 cites W2097265055 @default.
- W3035825831 cites W2097554174 @default.
- W3035825831 cites W2098100188 @default.
- W3035825831 cites W2099540110 @default.
- W3035825831 cites W2102690059 @default.
- W3035825831 cites W2114862447 @default.
- W3035825831 cites W2115522938 @default.
- W3035825831 cites W2117841435 @default.
- W3035825831 cites W2120660548 @default.
- W3035825831 cites W2123704230 @default.
- W3035825831 cites W2127394968 @default.
- W3035825831 cites W2127659175 @default.
- W3035825831 cites W2128443448 @default.
- W3035825831 cites W2131593425 @default.
- W3035825831 cites W2132250375 @default.
- W3035825831 cites W2135564059 @default.
- W3035825831 cites W2141365953 @default.
- W3035825831 cites W2143298565 @default.
- W3035825831 cites W2146848007 @default.
- W3035825831 cites W2153605484 @default.
- W3035825831 cites W2154140726 @default.
- W3035825831 cites W2160703969 @default.
- W3035825831 cites W2162252935 @default.
- W3035825831 cites W2163504716 @default.
- W3035825831 cites W2167279371 @default.
- W3035825831 cites W2167426126 @default.
- W3035825831 cites W2184699909 @default.
- W3035825831 cites W2343718859 @default.
- W3035825831 cites W2397227857 @default.
- W3035825831 cites W2601456187 @default.
- W3035825831 cites W2610732740 @default.
- W3035825831 cites W2619136378 @default.
- W3035825831 cites W2726161792 @default.
- W3035825831 cites W2753802561 @default.
- W3035825831 cites W2762533544 @default.
- W3035825831 cites W2765939774 @default.
- W3035825831 cites W2773293509 @default.
- W3035825831 cites W2779308139 @default.
- W3035825831 cites W2792244548 @default.
- W3035825831 cites W2809358976 @default.
- W3035825831 cites W2986040377 @default.
- W3035825831 cites W4211059758 @default.
- W3035825831 cites W4245481577 @default.
- W3035825831 cites W930075017 @default.
- W3035825831 doi "https://doi.org/10.1101/2020.06.22.164467" @default.
- W3035825831 hasPublicationYear "2020" @default.
- W3035825831 type Work @default.
- W3035825831 sameAs 3035825831 @default.
- W3035825831 citedByCount "2" @default.
- W3035825831 countsByYear W30358258312021 @default.
- W3035825831 countsByYear W30358258312023 @default.
- W3035825831 crossrefType "posted-content" @default.
- W3035825831 hasAuthorship W3035825831A5004905921 @default.
- W3035825831 hasAuthorship W3035825831A5008739379 @default.
- W3035825831 hasAuthorship W3035825831A5020529360 @default.
- W3035825831 hasAuthorship W3035825831A5030423855 @default.
- W3035825831 hasAuthorship W3035825831A5036408818 @default.
- W3035825831 hasAuthorship W3035825831A5049643687 @default.
- W3035825831 hasAuthorship W3035825831A5050356845 @default.
- W3035825831 hasAuthorship W3035825831A5066897632 @default.
- W3035825831 hasBestOaLocation W30358258311 @default.
- W3035825831 hasConcept C104317684 @default.