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- W3035826051 abstract "Crystalloids have been observed by light microscopy in Sertoli cells of both experimentally induced and spontaneous azoospermic testes of the swine and in Sertoli cells of normal human testes. However crystalloids have not been found in normal swine testes with the light microscope. Swine crystalloids are distinguished from human crystalloids in that the diameter of the filaments is 5 nm instead of 10-15 nm. This paper confirms this distinction by showing that the filaments of swine crystalloids bind heavy meromyosin (HMM) but those of human crystalloids do not. Human testes were removed from four patients with prostate cancer, whereas normal and cryptorchid swine testes were acquired from a slaughterhouse. HMM extracted from rabbit skeletal muscle was dissolved in 10 mM Tris buffer (pH 7. 4) containing 0. 1 M KCI and 0. 001 M MgCl 2 at a final concentration of 7 mg/ml. Pieces of testicular tissue were exposed to HMM at 4°C for 24 hours after glycerination or saponin treatment." @default.
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- W3035826051 date "1978-08-01" @default.
- W3035826051 modified "2023-10-17" @default.
- W3035826051 title "Crystalloids of actin-like filaments in the swine sertoli cell" @default.
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- W3035826051 doi "https://doi.org/10.1017/s0424820100083758" @default.
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