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- W3035851906 abstract "Scanning transmission electron microscopy (STEM) was used to study the structure of the extracellular hemoglobin of the annelid Lumbricus terrestris. These are giant molecules possessing a molecular weight of approximately 3.9 x 10 6 and appear in electron micrographs as a hexagonal bilayer, The samples for this study were diluted to 100 μg/ml with distilled water and applied to a thin ( ∽15 Å) carbon substrate supported on a microgrid. One percent (w/v) uranyl acetate solution was used for negative staining for 2 minutes and dried in air. STEM images were recorded using both a high resolution CRT with 1024 x 1024 picture elements and an on-line computer recording with 512 x 512 pixels. The images were rotated in on-line fashion with the computer to enhance contrast. The hemoglobin of Lumbricus assumes three common positions on the substrate (Fig. 1). The top view (Fig. la) demonstrates that the 1/12th subunits possess an approximately pentagonal shape with a central region of low protein density." @default.
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- W3035851906 date "1981-08-01" @default.
- W3035851906 modified "2023-09-25" @default.
- W3035851906 title "Scanning Transmission Electron Microscopy (STEM) of Annelid Extracellular Hemoglobins" @default.
- W3035851906 cites W2076073498 @default.
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- W3035851906 doi "https://doi.org/10.1017/s0424820100098666" @default.
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