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- W3035856811 abstract "Abstract The ectoine synthase EctC (EC 4.2.1.108) is a member of the hydro‐lyases (EC 4.2.1), a group of enzymes that cleaves carbon–oxygen bonds. It catalyzes the last step in the synthesis of the microbial osmotic stress‐protectant and chemical‐chaperone ectoine [( S )‐2‐methyl‐1,4,5,6‐tetrahydropyrimidine‐4‐carboxylic acid]. Genes encoding the ectoine biosynthetic enzymes are widely found in the members of the Bacteria but rarely in Archaea . Notably, a few halophilic protists and some marine microalgae are also capable of ectoine production. The EctC‐catalyzed enzyme reaction is strictly iron dependent and proceeds via cyclocondensation of the EctA‐formed substrate N ‐γ‐acetyl‐ l ‐2,4‐diaminobutyric acid via a water elimination. Crystal structures of the EctC proteins from the cold‐adapted Gram‐negative bacterium Sphingopyxis alaskensis ( Sa ) and the thermotolerant Gram‐positive bacterium Paenibacillus lautus ( Pl ) revealed a common overall protein fold and classify the ectoine synthase as a member of the cupin superfamily. The side chains of the three residues (Glu, Tyr, and His) coordinating the catalytically critical iron protrude into the lumen of the cupin barrel, closely positioned to the N ‐γ‐acetyl‐ l ‐2,4‐diaminobutyric acid substrate‐binding site. High‐resolution crystal structures of the ( Pl )EctC protein in its apo‐, substrate‐, and product‐bound forms allowed a proposal of the EctC enzyme reaction mechanism." @default.
- W3035856811 created "2020-06-25" @default.
- W3035856811 creator A5005825764 @default.
- W3035856811 creator A5024943759 @default.
- W3035856811 creator A5046618148 @default.
- W3035856811 creator A5077256764 @default.
- W3035856811 date "2020-06-19" @default.
- W3035856811 modified "2023-10-07" @default.
- W3035856811 title "Ectoine Synthase: An Iron‐Dependent Member of the Cupin Superfamily" @default.
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