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- W3035924897 abstract "After Albert Crewe et al. first detected single heavy atoms using a high resolution STEM in 1970, several enterprising microscopists atempted to use single heavy atoms as biological markers. The results were somewhat disappointing. Langmore found that specifically stained DNA rapidly lost its mercurial label, making sequencing this way impossible. Cole, Wiggins and Beer found that polynucleotides stained with osmium exhibited too much heavy atom motion to localize bases, but found that the osmium stayed with 5-10Å of the strands. Further work by Isaacson et al. quantified uranium atom motion at room temperature. It may be possible to reduce this atom motion by: 1) reducing the temperature, 2) reducing the beam dose, 3)better controlling the surface chemistry of the specimen or 4) using markers that do not move as much. The Brookhaven National Laboratory STEM was previously used to investigate possibility 1 and it was found that uranium atom motion was reduced by a factor of 2.2 in going from -40°C to -100°C." @default.
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- W3035924897 date "1978-01-01" @default.
- W3035924897 modified "2023-10-14" @default.
- W3035924897 title "A walking platinum complex" @default.
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- W3035924897 doi "https://doi.org/10.1017/s0424820100108386" @default.
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