Matches in SemOpenAlex for { <https://semopenalex.org/work/W3035996906> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3035996906 abstract "Abstract Caenorhabditis elegans is an excellent model organism for biological research, but its contributions to biochemical elucidation of eukaryotic transcription mechanisms have been limited. One of the biggest obstacles for biochemical studies of C. elegans is the high difficulty of preparing functionally active nuclear extract due to its thick surrounding cuticle. By employing Balch homogenization, we have achieved effective disruption of larval and adult worms and have obtained functionally active nuclear extract through subcellular fractionation. In vitro transcription reactions were successfully re-constituted using such nuclear extract. Furthermore, two non-radioactive detection methods, PCR and qRT-PCR, have been adapted into our system to qualitatively and quantitatively detect transcription, respectively. Using this system to assess how pathogen infection affects C. elegans transcription revealed that Pseudomonas aeruginosa infection increased transcription activity. Our in vitro system is useful for biochemically studying C. elegans transcription mechanisms and gene expression regulations. The effective preparation of functionally active nuclear extract in our system fills a technical gap in biochemical studies of C. elegans and will expand the usefulness of this model organism in addressing many biological questions beyond transcription." @default.
- W3035996906 created "2020-06-25" @default.
- W3035996906 creator A5001195635 @default.
- W3035996906 creator A5072850973 @default.
- W3035996906 creator A5088712927 @default.
- W3035996906 date "2020-06-23" @default.
- W3035996906 modified "2023-10-17" @default.
- W3035996906 title "A novel in vitro Caenorhabditis elegans transcription system" @default.
- W3035996906 cites W1486258091 @default.
- W3035996906 cites W19548065 @default.
- W3035996906 cites W1963688667 @default.
- W3035996906 cites W1979430872 @default.
- W3035996906 cites W1981148909 @default.
- W3035996906 cites W1993243592 @default.
- W3035996906 cites W2005222994 @default.
- W3035996906 cites W2021010187 @default.
- W3035996906 cites W2026344335 @default.
- W3035996906 cites W2053095874 @default.
- W3035996906 cites W2054306426 @default.
- W3035996906 cites W2085034589 @default.
- W3035996906 cites W2085527831 @default.
- W3035996906 cites W2086671458 @default.
- W3035996906 cites W2123785574 @default.
- W3035996906 cites W2149305968 @default.
- W3035996906 cites W2159565935 @default.
- W3035996906 cites W2518110374 @default.
- W3035996906 cites W2911367139 @default.
- W3035996906 cites W2967438276 @default.
- W3035996906 cites W2987767870 @default.
- W3035996906 cites W3022603721 @default.
- W3035996906 cites W4243859336 @default.
- W3035996906 cites W43799683 @default.
- W3035996906 cites W85750730 @default.
- W3035996906 doi "https://doi.org/10.1101/2020.06.22.149609" @default.
- W3035996906 hasPublicationYear "2020" @default.
- W3035996906 type Work @default.
- W3035996906 sameAs 3035996906 @default.
- W3035996906 citedByCount "0" @default.
- W3035996906 crossrefType "posted-content" @default.
- W3035996906 hasAuthorship W3035996906A5001195635 @default.
- W3035996906 hasAuthorship W3035996906A5072850973 @default.
- W3035996906 hasAuthorship W3035996906A5088712927 @default.
- W3035996906 hasBestOaLocation W30359969061 @default.
- W3035996906 hasConcept C104317684 @default.
- W3035996906 hasConcept C137858568 @default.
- W3035996906 hasConcept C138885662 @default.
- W3035996906 hasConcept C179926584 @default.
- W3035996906 hasConcept C19843653 @default.
- W3035996906 hasConcept C202751555 @default.
- W3035996906 hasConcept C2778944004 @default.
- W3035996906 hasConcept C41895202 @default.
- W3035996906 hasConcept C54355233 @default.
- W3035996906 hasConcept C70721500 @default.
- W3035996906 hasConcept C86339819 @default.
- W3035996906 hasConcept C86803240 @default.
- W3035996906 hasConcept C95444343 @default.
- W3035996906 hasConceptScore W3035996906C104317684 @default.
- W3035996906 hasConceptScore W3035996906C137858568 @default.
- W3035996906 hasConceptScore W3035996906C138885662 @default.
- W3035996906 hasConceptScore W3035996906C179926584 @default.
- W3035996906 hasConceptScore W3035996906C19843653 @default.
- W3035996906 hasConceptScore W3035996906C202751555 @default.
- W3035996906 hasConceptScore W3035996906C2778944004 @default.
- W3035996906 hasConceptScore W3035996906C41895202 @default.
- W3035996906 hasConceptScore W3035996906C54355233 @default.
- W3035996906 hasConceptScore W3035996906C70721500 @default.
- W3035996906 hasConceptScore W3035996906C86339819 @default.
- W3035996906 hasConceptScore W3035996906C86803240 @default.
- W3035996906 hasConceptScore W3035996906C95444343 @default.
- W3035996906 hasLocation W30359969061 @default.
- W3035996906 hasOpenAccess W3035996906 @default.
- W3035996906 hasPrimaryLocation W30359969061 @default.
- W3035996906 hasRelatedWork W11799287 @default.
- W3035996906 hasRelatedWork W14854171 @default.
- W3035996906 hasRelatedWork W15541922 @default.
- W3035996906 hasRelatedWork W19548065 @default.
- W3035996906 hasRelatedWork W23981381 @default.
- W3035996906 hasRelatedWork W25443891 @default.
- W3035996906 hasRelatedWork W28194013 @default.
- W3035996906 hasRelatedWork W33133445 @default.
- W3035996906 hasRelatedWork W8014543 @default.
- W3035996906 hasRelatedWork W8924676 @default.
- W3035996906 isParatext "false" @default.
- W3035996906 isRetracted "false" @default.
- W3035996906 magId "3035996906" @default.
- W3035996906 workType "article" @default.