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- W3036002642 abstract "In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli , under heat shock treatment are described. The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO 4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV." @default.
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- W3036002642 date "1992-08-01" @default.
- W3036002642 modified "2023-09-26" @default.
- W3036002642 title "Fish kidney cell line in response to heat shock" @default.
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- W3036002642 doi "https://doi.org/10.1017/s0424820100123921" @default.
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