Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036005698> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W3036005698 endingPage "S19" @default.
- W3036005698 startingPage "S19" @default.
- W3036005698 abstract "Background: The extensive involvement of microRNAs (miRNAs) in the pathogenesis of psoriasis is well documented. However, whether the serum exosome derived miRNAs play a role in psoriasis remains unclear. Objectives: To explore the role of serum exosome microRNA-1305 (miR-1305) in psoriasis. Methods: Exosomes were isolated from serum by differential ultracentrifugation. The morphology was identified by transmission electron microscope (TEM). Observation of serum exosomes uptake by keratinocytes though confocal fluorescence microscopy. MiRNA microarray was performed in serum exosomes from 4 patients with psoriasis and 4 controls. qRT-PCR were used to identify the differential expression miRNAs. Using bioinformatic predicted the signaling pathways related to mir-1305. Western blot was used to investigate the protein levels of Wnt5a and its downstream effectors. Normal human epidermal keratinocytes (NHEKs) and HaCat cells were transfected with miR-1305 mimic/Ctrl (50 nM) or miR-1305 inhibitor/Ctrl (100 nM); or siWnt5a/ctrl (60 nM) with Lipofectamine 2000. Results: The diameter of serum exosomes were around 100 nm. Serum exosomes could be uptake by keratinocytes after co-culture for 12h. Using miRNA microarray, we found 16 differentially expressed miRNAs in serum exosomes; 14 (87.5%) were downregulated and 2 (12.5%) were upregulated (fold change>2, P<0.05). Among these differentially expressed miRNAs, miR-1305 was down-regulated in serum exosomes from psoriasis patients (fold change=0.20, P<0.01, n=4). Through the methods of bioinformatics analysis, qRT-PCR and Western blot, we found that miR-1305 was closely related to non-canonical WNT signaling pathway (P<0.01), and miR-1305 regulated the expression of Wnt5a in Normal human epidermal keratinocytes (NHEKs) and HaCat cells. Conclusions: MiR-1305 was downregulated in serum exosomes from psoriasis patients. Downregulation of serum exosomes miR-1305 was involved in the pathogenesis of psoriasis through regulating non-canonical WNT pathways. However, much work remains to be done in the future to test our point of view." @default.
- W3036005698 created "2020-06-25" @default.
- W3036005698 creator A5013211825 @default.
- W3036005698 creator A5024391872 @default.
- W3036005698 date "2020-07-01" @default.
- W3036005698 modified "2023-09-30" @default.
- W3036005698 title "159 Downregulation of serum exosome miR-1305 contribute to the development of psoriasis through non-canonical Wnt signaling pathway" @default.
- W3036005698 doi "https://doi.org/10.1016/j.jid.2020.03.163" @default.
- W3036005698 hasPublicationYear "2020" @default.
- W3036005698 type Work @default.
- W3036005698 sameAs 3036005698 @default.
- W3036005698 citedByCount "0" @default.
- W3036005698 crossrefType "journal-article" @default.
- W3036005698 hasAuthorship W3036005698A5013211825 @default.
- W3036005698 hasAuthorship W3036005698A5024391872 @default.
- W3036005698 hasBestOaLocation W30360056981 @default.
- W3036005698 hasConcept C104317684 @default.
- W3036005698 hasConcept C127561419 @default.
- W3036005698 hasConcept C137620995 @default.
- W3036005698 hasConcept C145059251 @default.
- W3036005698 hasConcept C153911025 @default.
- W3036005698 hasConcept C203014093 @default.
- W3036005698 hasConcept C20518536 @default.
- W3036005698 hasConcept C2776415932 @default.
- W3036005698 hasConcept C2781261824 @default.
- W3036005698 hasConcept C502942594 @default.
- W3036005698 hasConcept C54355233 @default.
- W3036005698 hasConcept C62478195 @default.
- W3036005698 hasConcept C86803240 @default.
- W3036005698 hasConcept C95444343 @default.
- W3036005698 hasConceptScore W3036005698C104317684 @default.
- W3036005698 hasConceptScore W3036005698C127561419 @default.
- W3036005698 hasConceptScore W3036005698C137620995 @default.
- W3036005698 hasConceptScore W3036005698C145059251 @default.
- W3036005698 hasConceptScore W3036005698C153911025 @default.
- W3036005698 hasConceptScore W3036005698C203014093 @default.
- W3036005698 hasConceptScore W3036005698C20518536 @default.
- W3036005698 hasConceptScore W3036005698C2776415932 @default.
- W3036005698 hasConceptScore W3036005698C2781261824 @default.
- W3036005698 hasConceptScore W3036005698C502942594 @default.
- W3036005698 hasConceptScore W3036005698C54355233 @default.
- W3036005698 hasConceptScore W3036005698C62478195 @default.
- W3036005698 hasConceptScore W3036005698C86803240 @default.
- W3036005698 hasConceptScore W3036005698C95444343 @default.
- W3036005698 hasIssue "7" @default.
- W3036005698 hasLocation W30360056981 @default.
- W3036005698 hasOpenAccess W3036005698 @default.
- W3036005698 hasPrimaryLocation W30360056981 @default.
- W3036005698 hasRelatedWork W2039313867 @default.
- W3036005698 hasRelatedWork W2100692480 @default.
- W3036005698 hasRelatedWork W2594662887 @default.
- W3036005698 hasRelatedWork W3034307745 @default.
- W3036005698 hasRelatedWork W3086820975 @default.
- W3036005698 hasRelatedWork W3157283225 @default.
- W3036005698 hasRelatedWork W3157625596 @default.
- W3036005698 hasRelatedWork W3189527463 @default.
- W3036005698 hasRelatedWork W3210445590 @default.
- W3036005698 hasRelatedWork W4323659723 @default.
- W3036005698 hasVolume "140" @default.
- W3036005698 isParatext "false" @default.
- W3036005698 isRetracted "false" @default.
- W3036005698 magId "3036005698" @default.
- W3036005698 workType "article" @default.