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- W3036011509 abstract "Multiple sectioning: Two or more core specimens from a kidney needle biopsy can be embedded in single block using an 8-facet silicone rubber embedding mold. Tissue blocks are softened by placing them face down on an 85°C hot plate for one minute and then trimming with a Teflon coated razor blade. This prevents the block face from being deeply fractured which can affect section quality. If glomeruli are found within different cores in a single block, the block face can be retrimmed into multiple mesas and simultaneously thin-sectioned. This method greatly increases the efficiency of both thick and thin sectioning which is extremely helpful when handling more than 1600 cases of renal biopsies in our EM laboratory each year. Knife Spacer: Due to the difference in thickness between a glass and diamond knife, and also depending on which portion of the diamond knife is being used, the knife stage has to be moved laterally over a long distance when changing from one knife type to the other. Placing a L-shaped spacer, 3 to 5 mm in thickness, on the left side of the stage when sectioning a glass knife will greatly reduce this lateral adjustment." @default.
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- W3036011509 date "1996-08-11" @default.
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- W3036011509 title "Practical methods for TEM" @default.
- W3036011509 doi "https://doi.org/10.1017/s0424820100165604" @default.
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