Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036148745> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W3036148745 abstract "ABSTRACT Background Escherichia coli O157 is an emerging foodborne pathogen of great public health concern. It has been associated with bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome in humans. Most human infections have been traced to cattle and the consumption of contaminated cattle products. In order to understand the risk associated with the consumption of cattle products, this study sought to investigate the prevalence and identify virulence genes in E. coli O157 from cattle in Cameroon. Method A total of 512 rectal samples were obtained and analysed using conventional bacteriological methods (enrichment on modified Tryptone Soy Broth and selective plating on Cefixime-Tellurite Sorbitol Mac-Conkey Agar) for the isolation of E. coli O157. Presumptive E. coli O157 isolates were confirmed serologically using E. COLIPRO ™ O157 latex agglutination test and molecularly using PCR targeting the rfb gene in the isolates. Characterisation of the confirmed E. coli O157 strains was done by amplification of stx1 , stx2 , eaeA and hlyA virulence genes using both singleplex and multiplex PCR. Results E. coli O157 was detected in 56 (10.9%) of the 512 samples examined. The presence of the virulence genes stx2, eaeA and hylA was demonstrated in 96.4% (54/56) of the isolates and stx1 in 40 (71.4%) of the 54. The isolates exhibited three genetic profiles (I-III) with I ( stx1, stx2, eaeA and hlyA ) being the most prevalent (40/56; 71.4%) while two isolates had none of the virulence genes tested. Conclusion A proportion of cattle slaughtered in abattoirs in Buea are infected with pathogenic E. coli O157 and could be a potential source of human infections. We recommend proper animal food processing measures and proper hygiene be prescribed and implemented to reduce the risk of beef contamination." @default.
- W3036148745 created "2020-06-25" @default.
- W3036148745 creator A5042032179 @default.
- W3036148745 date "2020-06-19" @default.
- W3036148745 modified "2023-09-27" @default.
- W3036148745 title "Prevalence and Virulence Gene Profiles of Escherichia coli O157 from Cattle Slaughtered in Buea, Cameroon" @default.
- W3036148745 cites W1538707760 @default.
- W3036148745 cites W155690413 @default.
- W3036148745 cites W1860937064 @default.
- W3036148745 cites W1988807794 @default.
- W3036148745 cites W2003915689 @default.
- W3036148745 cites W2016786514 @default.
- W3036148745 cites W2026107123 @default.
- W3036148745 cites W2051000559 @default.
- W3036148745 cites W2056700308 @default.
- W3036148745 cites W2060797304 @default.
- W3036148745 cites W2063547978 @default.
- W3036148745 cites W2074816988 @default.
- W3036148745 cites W2079285076 @default.
- W3036148745 cites W2082661031 @default.
- W3036148745 cites W2103444887 @default.
- W3036148745 cites W2103798600 @default.
- W3036148745 cites W2105383327 @default.
- W3036148745 cites W2115701449 @default.
- W3036148745 cites W2120919686 @default.
- W3036148745 cites W2133831708 @default.
- W3036148745 cites W2159227713 @default.
- W3036148745 cites W2161186278 @default.
- W3036148745 cites W2164129442 @default.
- W3036148745 cites W2168415674 @default.
- W3036148745 cites W2314870262 @default.
- W3036148745 cites W659444774 @default.
- W3036148745 cites W994913268 @default.
- W3036148745 doi "https://doi.org/10.1101/2020.06.19.161166" @default.
- W3036148745 hasPublicationYear "2020" @default.
- W3036148745 type Work @default.
- W3036148745 sameAs 3036148745 @default.
- W3036148745 citedByCount "0" @default.
- W3036148745 crossrefType "posted-content" @default.
- W3036148745 hasAuthorship W3036148745A5042032179 @default.
- W3036148745 hasBestOaLocation W30361487451 @default.
- W3036148745 hasConcept C104317684 @default.
- W3036148745 hasConcept C159654299 @default.
- W3036148745 hasConcept C2777399836 @default.
- W3036148745 hasConcept C2778411228 @default.
- W3036148745 hasConcept C2778660310 @default.
- W3036148745 hasConcept C2781001907 @default.
- W3036148745 hasConcept C37977956 @default.
- W3036148745 hasConcept C49105822 @default.
- W3036148745 hasConcept C523546767 @default.
- W3036148745 hasConcept C54355233 @default.
- W3036148745 hasConcept C547475151 @default.
- W3036148745 hasConcept C60987743 @default.
- W3036148745 hasConcept C86803240 @default.
- W3036148745 hasConcept C89423630 @default.
- W3036148745 hasConcept C90583042 @default.
- W3036148745 hasConceptScore W3036148745C104317684 @default.
- W3036148745 hasConceptScore W3036148745C159654299 @default.
- W3036148745 hasConceptScore W3036148745C2777399836 @default.
- W3036148745 hasConceptScore W3036148745C2778411228 @default.
- W3036148745 hasConceptScore W3036148745C2778660310 @default.
- W3036148745 hasConceptScore W3036148745C2781001907 @default.
- W3036148745 hasConceptScore W3036148745C37977956 @default.
- W3036148745 hasConceptScore W3036148745C49105822 @default.
- W3036148745 hasConceptScore W3036148745C523546767 @default.
- W3036148745 hasConceptScore W3036148745C54355233 @default.
- W3036148745 hasConceptScore W3036148745C547475151 @default.
- W3036148745 hasConceptScore W3036148745C60987743 @default.
- W3036148745 hasConceptScore W3036148745C86803240 @default.
- W3036148745 hasConceptScore W3036148745C89423630 @default.
- W3036148745 hasConceptScore W3036148745C90583042 @default.
- W3036148745 hasLocation W30361487451 @default.
- W3036148745 hasLocation W30361487452 @default.
- W3036148745 hasOpenAccess W3036148745 @default.
- W3036148745 hasPrimaryLocation W30361487451 @default.
- W3036148745 hasRelatedWork W1985930089 @default.
- W3036148745 hasRelatedWork W1994145981 @default.
- W3036148745 hasRelatedWork W2141071177 @default.
- W3036148745 hasRelatedWork W2316632767 @default.
- W3036148745 hasRelatedWork W2620852090 @default.
- W3036148745 hasRelatedWork W3036148745 @default.
- W3036148745 hasRelatedWork W3039683749 @default.
- W3036148745 hasRelatedWork W3209908485 @default.
- W3036148745 hasRelatedWork W78296084 @default.
- W3036148745 hasRelatedWork W2186430898 @default.
- W3036148745 isParatext "false" @default.
- W3036148745 isRetracted "false" @default.
- W3036148745 magId "3036148745" @default.
- W3036148745 workType "article" @default.