FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036193054> ?p ?o ?g. }

• W3036193054 abstract "Eukaryotic histone methyltransferases 2 (EHMT2 or G9A) has been regarded as a potential target for non-small cell lung cancer (NSCLC) therapy. This study investigated the regulatory roles of G9A in tumorigenesis and stemness in NSCLC. We isolated and enriched tumor-initiating cells (TIC) from surgically resected NSCLC tissues by FACS and sphere formation assays. We then knocked down G9A using shRNA and carried out genome-wide 850K methylation array and RNA sequencing analyses. We carried out in vivo tumorigenecity asssay using mice xenografts and examined G9A interactions with its novel target using chromatin Immunoprecipitation (ChIP).We identified 67 genes hypomethylated and 143 genes upregulated following G9A knockdown of which 43 genes were both hypomethylated and upregulated. We selected six genes (CDYL2, DPP4, SP5, FOXP1, STAMBPL1, and ROBO1) for validation. In addition, G9A expression was higher in TICs and targeting G9a by shRNA knockdown or by selective inhibitor UNC0642 significantly inhibited the expression of cancer stem cell markers and sphere forming capacity, in vitro proliferation, and in vivo growth. Further, transient overexpression of FOXP1, a protein may promote normal stem cell differentiation, in TICs resulted in downregulation of stem cell markers and sphere forming capacity and cell proliferation in vitro indicating that the genes we identified are directly regulated by G9A through aberrant DNA methylation and subsequent expression. Similarly, ChIP assay has shown that G9a interacts with its target genes through H3K9me2 and downregulation of H3K9me2 following G9a knockdown disrupts its interaction with its target genes.These data suggest that G9A is involved in lung cancer stemness through epigenetic mechanisms of maintaining DNA methylation of multiple lung cancer stem cell genes and their expression. Further, targeting G9A or its downstream genes could be a novel therapeutic approach in treating NSCLC patients." @default.
• W3036193054 created "2020-06-25" @default.
• W3036193054 creator A5009470333 @default.
• W3036193054 creator A5019854401 @default.
• W3036193054 creator A5022765898 @default.
• W3036193054 creator A5023989141 @default.
• W3036193054 creator A5029393895 @default.
• W3036193054 creator A5037590196 @default.
• W3036193054 creator A5040658425 @default.
• W3036193054 creator A5047899047 @default.
• W3036193054 creator A5052704860 @default.
• W3036193054 creator A5088048815 @default.
• W3036193054 date "2020-06-17" @default.
• W3036193054 modified "2023-10-16" @default.
• W3036193054 title "G9a regulates tumorigenicity and stemness through genome-wide DNA methylation reprogramming in non-small cell lung cancer" @default.
• W3036193054 cites W1564613369 @default.
• W3036193054 cites W1911726069 @default.
• W3036193054 cites W1920711459 @default.
• W3036193054 cites W1921414899 @default.
• W3036193054 cites W1968263601 @default.
• W3036193054 cites W1973193187 @default.
• W3036193054 cites W1978810080 @default.
• W3036193054 cites W1981303601 @default.
• W3036193054 cites W1989831120 @default.
• W3036193054 cites W2021459974 @default.
• W3036193054 cites W2034546452 @default.
• W3036193054 cites W2036734716 @default.
• W3036193054 cites W2045399385 @default.
• W3036193054 cites W2059999434 @default.
• W3036193054 cites W2070300060 @default.
• W3036193054 cites W2072202929 @default.
• W3036193054 cites W2083274861 @default.
• W3036193054 cites W2084705909 @default.
• W3036193054 cites W2085667285 @default.
• W3036193054 cites W2085934079 @default.
• W3036193054 cites W2089818916 @default.
• W3036193054 cites W2099540110 @default.
• W3036193054 cites W2105624032 @default.
• W3036193054 cites W2112702770 @default.
• W3036193054 cites W2114104545 @default.
• W3036193054 cites W2121655261 @default.
• W3036193054 cites W2126496132 @default.
• W3036193054 cites W2134526812 @default.
• W3036193054 cites W2135190835 @default.
• W3036193054 cites W2142373051 @default.
• W3036193054 cites W2150782426 @default.
• W3036193054 cites W2150963367 @default.
• W3036193054 cites W2154471713 @default.
• W3036193054 cites W2158627893 @default.
• W3036193054 cites W2159778357 @default.
• W3036193054 cites W2169456326 @default.
• W3036193054 cites W2180481128 @default.
• W3036193054 cites W2255672707 @default.
• W3036193054 cites W2275877493 @default.
• W3036193054 cites W2295352084 @default.
• W3036193054 cites W2346025119 @default.
• W3036193054 cites W2364794827 @default.
• W3036193054 cites W2408912897 @default.
• W3036193054 cites W2512421145 @default.
• W3036193054 cites W2518279214 @default.
• W3036193054 cites W2582838961 @default.
• W3036193054 cites W2587862832 @default.
• W3036193054 cites W2727290711 @default.
• W3036193054 cites W2737272162 @default.
• W3036193054 cites W2743506403 @default.
• W3036193054 cites W2753207486 @default.
• W3036193054 cites W2754970987 @default.
• W3036193054 cites W2758811376 @default.
• W3036193054 cites W2763367287 @default.
• W3036193054 cites W2763583346 @default.
• W3036193054 cites W2767609334 @default.
• W3036193054 cites W2770230492 @default.
• W3036193054 cites W2776430148 @default.
• W3036193054 cites W2781470301 @default.
• W3036193054 cites W2784371446 @default.
• W3036193054 cites W2809404378 @default.
• W3036193054 cites W2895790180 @default.
• W3036193054 cites W2898995534 @default.
• W3036193054 cites W2903301829 @default.
• W3036193054 cites W2906638098 @default.
• W3036193054 cites W2907262475 @default.
• W3036193054 cites W2910248442 @default.
• W3036193054 cites W2911188335 @default.
• W3036193054 cites W2911982425 @default.
• W3036193054 cites W2947538095 @default.
• W3036193054 cites W2949114698 @default.
• W3036193054 cites W2952290231 @default.
• W3036193054 cites W2954137797 @default.
• W3036193054 cites W2969012569 @default.
• W3036193054 cites W4363260 @default.
• W3036193054 doi "https://doi.org/10.1186/s13148-020-00879-5" @default.
• W3036193054 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7302379" @default.
• W3036193054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32552834" @default.
• W3036193054 hasPublicationYear "2020" @default.
• W3036193054 type Work @default.
• W3036193054 sameAs 3036193054 @default.
• W3036193054 citedByCount "24" @default.
• W3036193054 countsByYear W30361930542020 @default.
• W3036193054 countsByYear W30361930542021 @default.
• W3036193054 countsByYear W30361930542022 @default.

• next