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- W3036200068 abstract "The application of transmission electron microscopy (TEM) has greatly increased our understanding of structure-function relationships in bacteriology. However, to achieve further advancements investigators are seeking preparation procedures that would avoid the artifacts associated with conventional chemical fixation, dehydration and critical point drying or embedding. In our laboratory a field emission scanning electron microscope (SEM) was recently equipped with a cold stage. This combination of techniques, referred to as low temperature (LT) SEM, allowed us to examine frozen, fully hydrated biological specimens. The present investigation images bacteria that were cryofixed for LTSEM observations and then freeze-substituted for TEM observations. In addition an attempt was made to culture the samples that had been cryofixed and observed with LTSEM so that taxonomic identification and further microscopic observations could be made. Bacteria used in this study were isolated from spinach leaves (variety New Jersey). LTSEM observations of cryofixed samples were performed on a Hitachi S-4100 field emission SEM equipped with an Oxford CT-1500HF Cryotrans System." @default.
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- W3036200068 date "1995-08-13" @default.
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- W3036200068 title "Advantages of Low Temperature SEM for Bacterial Studies" @default.
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- W3036200068 doi "https://doi.org/10.1017/s042482010014172x" @default.
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