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- W3036204543 abstract "Water in biological specimens, when cooled at a very high rate can be immobilized in a metastable, amorphous, so-called vitreous state. Kept vitreous at temperatures below 130 K, the vapour presure of water is so low, that the specimens will not dry when observed directly in the EM. Therefore, cryo-EM of vitrified biological specimens ommits artifacts due to dehydration and chemical fixation. One field of interest in the application of this technique is the study of the structural organisation of genomic material in situ. The objects to be studied, i.e. isolated nuclei, culture cells, or tissues are all too large to allow investigation as a whole in the TEM; they must be observed as ultrathin sections. While the cutting of vitrified bulk specimens into ultrathin sections is possible, the cutting process provokes severe deformation: The sections are compressed along the cutting direction by ca 50% and correspondingly, the section thickness doubles in respect to the actual advance of the microtome, which indicates important displacements of material takeing place during sectioning. Nevertheless structural information even in small range order can be conserved." @default.
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- W3036204543 date "1990-08-12" @default.
- W3036204543 modified "2023-10-18" @default.
- W3036204543 title "High-resolution Study of DNA in Vitrified Sections" @default.
- W3036204543 cites W2103972113 @default.
- W3036204543 doi "https://doi.org/10.1017/s0424820100181191" @default.
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