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- W3036217498 abstract "The human immunodeficiency virus (HIV-1) is dependent upon the synthesis of TAT for efficient transcriptional activation and replication. Following translocation to the nucleus TAT interacts with a cis-acting TAT-responsive element located between nucleotides +1 to +42 within the HIV- 1 long terminal repeat. This region contains an RNA stem loop structure TAR, which interacts with TAT and cellular proteins to increase transcription 1000 fold. We have transfected human T-lymphocytes with an expression vector containing the HIV-1 LTR driving the synthesis of TAT-72. A stable cell line was generated by selection with G418. Cells were grown on round glass coverslips coated with a solution of fibronectin overnight (at 37°C). In addition, cells on coverslips were fixed with a 3.2% glutaraldehyde buffered solution (0.1M Na cacodylate) for 5 min at room temperature, then 15 min at 4°C, then washed gently in a buffer-sucrose solution. Postfixation was performed with an aqueous mixture containing 1.5% OsO 4 + 0.5% RuO 4 for 20 min at 18°C." @default.
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- W3036217498 date "1995-08-13" @default.
- W3036217498 modified "2023-09-24" @default.
- W3036217498 title "Human T-lymphocytes (Jurkat cells) expressing HIV-1 TAT-72: SEM" @default.
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- W3036217498 doi "https://doi.org/10.1017/s0424820100141445" @default.
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