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- W3036233753 abstract "Post-embedding immunogold labelling (IEM) can provide an exact correlation between the ultrastructure of tumor cells and the location of specific antigens. This technology links the molecular features of tumor cells derived from immunohistochemistry with our knowledge of tumor ultrastructure from electron microscopy. Criteria for the use of IEM in the study of human tissues include: the ability to localize many different antigens with commercially available antibodies; specimen preparation that is rapid, standardized and effective for different biopsies and tissue culture; results should compare with immunoperoxidase labelling on formalin-fixed, paraffin-embedded tissue. The tumors examined in this study included: squamous carcinomas, melanomas, multiple myelomas, schwannomas, medullary carcinomas of the thyroid, adenocarcinomas, leiomyomas, germ-cell carcinoma, myeloid leukemia, lymphomas, merkel cell carcinoma, and carcinoid tumors. Fine-needle aspiration biopsies (FNAB), core biopsies of bone marrow, peripheral blood, cell culture monolayers and surgically removed tissues were fixed in 1.6% glutaraldehyde. Samples were dehydrated, infiltrated in Lowicryl K4M and UV polymerized (total preparation time: 3 days). Technique for FNAB samples used mesh filtration to purify tumor cells which were then entrapped in albumin prior to embedding to prevent loss of cells. Glass coverslips (4x2mm) bearing cell monolayers were fixed in situ , infiltrated, placed in gelatin capsules and the cells separated from the coverslips after polymerization for en face sectioning. Thin sections were immunolabelled with readily available monoclonal and polyclonal antibodies using the protein A-gold technique. Samples of normal tissues were labelled for each antigen as positive controls and the negative controls used had appropriate low levels of non-specific labelling." @default.
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- W3036233753 date "1989-08-06" @default.
- W3036233753 modified "2023-09-27" @default.
- W3036233753 title "Applications of post-embedding immunogold labelling in the study of human tumors" @default.
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- W3036233753 doi "https://doi.org/10.1017/s0424820100156365" @default.
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