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- W3036288762 abstract "A previous light microscopic study determined that ~70% of all connective tissue mast cells (CTMCs) in the thalamus of the rat were adjacent to periodic acid-Schiff staining cells which also were ED2 positive therefore identified as a type of macrophage. Using transmission electron microscopy, the goal of the present study was to examine that association at the ultrastructural level and to determine the relationship of each of these cells to other components of the cerebrovasculature. Brains of anesthetized adult male Sprague-Dawley rats were fixed by intracardiac perfusion with paraformaldehyde and glutaraldehyde [2%:2.5% (phosphate buffer 0.1 M, pH 7.40 or 2%:2.5% for 5 min followed by 1%: 1.25% (phosphate buffer 80 mM, pH 7.4) for the remainder of the fixation]. Brains were sliced into ~1 mm sections and samples of thalamus were postfixed in OSO 4 (1%, 0.1 M phosphate buffer), dehydrated, and flat embedded in LX112. Semi-thin (1 μm) and thin (60-90 nm) sections were cut and stained with toluidine blue and uranyl acetate/lead citrate respectively. Grids were viewed on a JOEL 100CXII electron microscope at 80kV." @default.
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- W3036288762 date "1995-08-13" @default.
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- W3036288762 title "Ultrastructural analysis redefines light microscopic findings relative to morphological relationships among cells of the cerebral vasculature in the rat" @default.
- W3036288762 doi "https://doi.org/10.1017/s0424820100141147" @default.
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