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- W3036351628 abstract "Nonmammalian models for investigations involving carcinogenesis have been developed as complementary extensions of current mammalian testing systems. Several species of fish, including Japanese medaka Oryzias latipes have proven to be very effective and reliable models for carcinogenesis studies, the majority of which have concentrated on hepatocellular carcinogens. The primary objective of this investigation was to provide basic ultrastructural information regarding cultured liver cells of medaka which would be beneficial in subsequent experiments relating to the utilization of cell cultures in carcinogenesis studies. Primary cultures of cells from livers of 20 adult medaka were obtained by trypsinization of fragmented organs. Initial cell viability, as monitored by trypan blue exclusion, was determined to be greater than 95%. Cells were plated in a complete medium composed of Dulbecco's MEM supplemented with 10% fetal calf serum, 25mM HEPES buffer, 1% sodium pyruvate, 1% non-essential amino acids and antibodies. Cell density of 5.0 to 7.5x10 5 cells/ml was optimal." @default.
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- W3036351628 date "1991-08-01" @default.
- W3036351628 modified "2023-10-18" @default.
- W3036351628 title "Ultrastructural characterization of primary cultures of liver cells obtained from the Japanese medaka, Oryzias latipe" @default.
- W3036351628 cites W1978630371 @default.
- W3036351628 doi "https://doi.org/10.1017/s0424820100084995" @default.
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