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- W303639258 abstract "Cytogenetic studies of peripheral lymphocytes were initiated on a couple with a history of three spontaneous 1st trimester losses and oligospermia. The results revealed the woman to have a normal female karyotype. The man had a karyotype of 46,XY,inv(2)(q14.2q24.3). Interphase sperm studies were offered as an attempt to quantify the relative proportion of sperm with acentric and dicentric chromosome No. 2 in response to the couple`s concern that chromosomally unbalanced sperm resulting from recombination of the inversion was the primary cause of pregnancy losses. Slides were prepared and processed as previously described. A two-color probe cocktail consisting of a biotinylated {alpha}-satellite probe for No. 2 and a digoxigenin-labeled probe for {alpha}-satellite No. 17 as an internal control was employed. Among 496 cells with a single signal for chromosome No. 17, we observed 2 with no signal for chromsome No. 2, 492 with a single signal for No. 2, and 2 cells with 2 signals for No. 2. These findings indicate that the proportion of unbalanced recombinant sperm is probably under 1%, and that other factors are likely to be involved in the etiology of this couple`s pregnancy losses." @default.
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- W303639258 date "1994-09-01" @default.
- W303639258 modified "2023-09-28" @default.
- W303639258 title "Interphase study by fluorescence in situ hybridization of spermatozoa of a paracentric inversion heterozygote" @default.
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