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- W3036404666 abstract "In the mouse, mast cell progenitor cells (Thyl +) have been shown to originate in the bone marrow. In humans, it has been suggested that mast cell progenitors also exist in the bone marrow, are derived from a common stem cell, and mature under cytokine influence in the tissue. Progenitor cells or CD34+ cells comprise approximately 1% of bone marrow mononuclear cells. Previously we have shown that a heterogenous population of human bone marrow cells cultured over agarose surfaces in the presence of rhIL-3 gives rise to basophils and small numbers of mast cells. Cells were identified as basophils based on characteristic morphology, metachromatic staining with Wright-Giemsa and acid toluidine blue, surface IgE as determined by fluorescence and the presence of histamine by o-Phthaldialdehyde condensation. These cells did not stain for the mast cell specific neutral protease, tryptase. Cells identified as mast cells had IgE receptors, contained histamine and were chloroacetate esterase and human mast cell tryptase positive." @default.
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- W3036404666 date "1990-08-12" @default.
- W3036404666 modified "2023-09-26" @default.
- W3036404666 title "Ultrastructural characterization of basophils and mast cells derived from CD34+ human bone marrow progenitor cells" @default.
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- W3036404666 doi "https://doi.org/10.1017/s042482010015931x" @default.
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