Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036459593> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W3036459593 abstract "This work presents two-photon polymerization (2PP) of microstructured 3D hydrogels using alginate for the first time. Alginate is one of the most prevalent biopolymersused for hydrogels due to its biocompatibility, reversible ionic gelation and natural abundance. 2PP enables the fabrication of structures with sub-micron feature size,on the scale of single cells. Combining the high resolution of 2PP with the hydrogel properties of alginate, enables control of the mechanical and geometrical properties of artificial micro-environments.To provide a curing mechanism using 2PP, alginate is functionalised with methacrylate groups using an amidation reaction. The 2PP photoresist is composed of methacrylatedalginate dissolved in water and a cyclic benzylidene ketone-based photoinitiator, P2CK, developed for efficient 2PP of biocompatible water-soluble photoresists.To investigate the 2PP characteristics of methacrylated alginate, various photoresist compositions were systematically studied in relation to the alginate concentration, the viscosity and the degree of functionalisation with crosslinking groups of alginate. This revealed that higher degrees of functionalised alginates featured lower polymerization but higher damage-threshold powers resulting in the largest dynamic power ranges. Increasing concentrations of alginate reduced the deformation of all alginate hydrogels.Stable 3D hydrogels could be fabricated using at least two weight percent for alginates functionalised with 30 percent or more methacrylate groups. Optimal printing parameters were identified for alginate photoresists that enable efficient 2PP of hydrogels with high structural integrity. An unprecedented resolution for alginate hydrogels was demonstrated with minimal linewidths down to 175 nm for two weight percent alginate with 30 percent functional groups. The alginate hydrogels were probed for potential cytotoxic effects on bacterial and mammalian cells. A representative for Gram-negative and a Gram-positive bacteria grown on a set of different micro-topographies resulted in distinct differences of bacterial attachment which could be exploited to prevent bacterial growth. The microfabricated alginate hydrogels were further shown to support the growth of human mesenchymal stem cells (hMSCs) with first results indicating that microstructures influence the attachment and morphology of hMSCs cultured for 48 hours. 2PP of alginate allows for precisely engineering artificial cell environments whichmay enable novel tissue engineering strategies and microbiology investigations. The increased understanding of the variables influencing 2PP of alginate will inform and accelerate the development of other novel water-based photoresists for 2PP." @default.
- W3036459593 created "2020-06-25" @default.
- W3036459593 creator A5081804904 @default.
- W3036459593 date "2020-03-15" @default.
- W3036459593 modified "2023-09-24" @default.
- W3036459593 title "Two-photon polymerization of alginate hydrogels for applications in cell culture" @default.
- W3036459593 hasPublicationYear "2020" @default.
- W3036459593 type Work @default.
- W3036459593 sameAs 3036459593 @default.
- W3036459593 citedByCount "0" @default.
- W3036459593 crossrefType "dissertation" @default.
- W3036459593 hasAuthorship W3036459593A5081804904 @default.
- W3036459593 hasConcept C108586683 @default.
- W3036459593 hasConcept C127413603 @default.
- W3036459593 hasConcept C132050475 @default.
- W3036459593 hasConcept C134406635 @default.
- W3036459593 hasConcept C159985019 @default.
- W3036459593 hasConcept C166940927 @default.
- W3036459593 hasConcept C171250308 @default.
- W3036459593 hasConcept C185592680 @default.
- W3036459593 hasConcept C188027245 @default.
- W3036459593 hasConcept C191897082 @default.
- W3036459593 hasConcept C192562407 @default.
- W3036459593 hasConcept C2777230088 @default.
- W3036459593 hasConcept C2779227376 @default.
- W3036459593 hasConcept C2780634384 @default.
- W3036459593 hasConcept C42360764 @default.
- W3036459593 hasConcept C44228677 @default.
- W3036459593 hasConcept C521977710 @default.
- W3036459593 hasConceptScore W3036459593C108586683 @default.
- W3036459593 hasConceptScore W3036459593C127413603 @default.
- W3036459593 hasConceptScore W3036459593C132050475 @default.
- W3036459593 hasConceptScore W3036459593C134406635 @default.
- W3036459593 hasConceptScore W3036459593C159985019 @default.
- W3036459593 hasConceptScore W3036459593C166940927 @default.
- W3036459593 hasConceptScore W3036459593C171250308 @default.
- W3036459593 hasConceptScore W3036459593C185592680 @default.
- W3036459593 hasConceptScore W3036459593C188027245 @default.
- W3036459593 hasConceptScore W3036459593C191897082 @default.
- W3036459593 hasConceptScore W3036459593C192562407 @default.
- W3036459593 hasConceptScore W3036459593C2777230088 @default.
- W3036459593 hasConceptScore W3036459593C2779227376 @default.
- W3036459593 hasConceptScore W3036459593C2780634384 @default.
- W3036459593 hasConceptScore W3036459593C42360764 @default.
- W3036459593 hasConceptScore W3036459593C44228677 @default.
- W3036459593 hasConceptScore W3036459593C521977710 @default.
- W3036459593 hasLocation W30364595931 @default.
- W3036459593 hasOpenAccess W3036459593 @default.
- W3036459593 hasPrimaryLocation W30364595931 @default.
- W3036459593 hasRelatedWork W1904424573 @default.
- W3036459593 hasRelatedWork W2014399554 @default.
- W3036459593 hasRelatedWork W2038659647 @default.
- W3036459593 hasRelatedWork W2047467106 @default.
- W3036459593 hasRelatedWork W2060205037 @default.
- W3036459593 hasRelatedWork W2072473321 @default.
- W3036459593 hasRelatedWork W2135990786 @default.
- W3036459593 hasRelatedWork W2804477276 @default.
- W3036459593 hasRelatedWork W2807811066 @default.
- W3036459593 hasRelatedWork W2890859031 @default.
- W3036459593 hasRelatedWork W2910355745 @default.
- W3036459593 hasRelatedWork W2976030955 @default.
- W3036459593 hasRelatedWork W3000722546 @default.
- W3036459593 hasRelatedWork W3048910143 @default.
- W3036459593 hasRelatedWork W3105255083 @default.
- W3036459593 hasRelatedWork W3141861096 @default.
- W3036459593 hasRelatedWork W3175405050 @default.
- W3036459593 hasRelatedWork W3197120783 @default.
- W3036459593 hasRelatedWork W3207007070 @default.
- W3036459593 hasRelatedWork W2566937284 @default.
- W3036459593 isParatext "false" @default.
- W3036459593 isRetracted "false" @default.
- W3036459593 magId "3036459593" @default.
- W3036459593 workType "dissertation" @default.