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- W3036509318 abstract "We have developed CRISPR-assisted RNA-protein interaction detection method (CARPID), which leverages CRISPR-CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs) in the native cellular context. We applied CARPID to the nuclear lncRNA XIST, and it captured a list of known interacting proteins and multiple previously uncharacterized binding proteins. We generalized CARPID to explore binders of the lncRNAs DANCR and MALAT1, revealing the method's wide applicability in identifying RNA-binding proteins." @default.
- W3036509318 created "2020-06-25" @default.
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- W3036509318 date "2020-06-22" @default.
- W3036509318 modified "2023-10-06" @default.
- W3036509318 title "CRISPR-assisted detection of RNA–protein interactions in living cells" @default.
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- W3036509318 doi "https://doi.org/10.1038/s41592-020-0866-0" @default.
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