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- W3036539226 abstract "AbstractThis study was conducted on seven pistachio cultivars to examine the effect of storage conditions on pollen viability and in vitro pollen germination under different durations. Each pollen was stored in room temperature (24 oC±2), refrigerator (4oC) and freezer (-5oC) for 0, 1, 2, 3 and 4 weeks. Pollen viability was investigated by using staining methods including TTC, IKI and safranine solutions. The results showed that at all storage methods and periods, pollen viability and in vitro pollen germination were significantly the highest for Batouri and Ashouri cultivars and the lowest for Marawhi and Elemi cultivars. The results showed that the highest pollen viability was attained by safranin when pollen stored under freezer condition. However, pollen viability by TTC was the lowest at room temperature storage. In addition, in vitro pollen germination and viability were decreased significantly as storage period increases. This study revealed no differences between in vitro germination percentages for refrigerated and frozen stored pollen up to 2 weeks. Meanwhile, in vitro germination of room stored pollen was gradually decreased when storage duration increased. At the end of storage period, pollen viability was reduced slightly under freezer conditions whereas the reduction in viability was the largest for refrigerated and room stored pollen with no differences between them. This experiment showed a significant cultivar x storage method x period interaction for pollen viability but not for in vitro pollen germination. Pistachio (Pistacia vera L.) is an individual from the family Anacardiaceae (Ak et al., 2016). Pistachio trees are dioecious, implying that the pistillate and staminate blossoms are shaped on various trees. Pistachio trees are wind pollinated as blossoms have no petals to pull in creepy crawlies (Hosseini et al., 2015). One male tree is required for each eight female trees, yet this proportion is generally not seen in plantations (Bahramabadi et al., 2018). In this way, the measure of dust delivered in each bunch and germination pace of dust must be high in male trees (Ak et al., 2016). Pistachio is basically developed in warm-calm to subtropical pieces of the world for its economically important and palatable seeds (Tilkat and Onay, 2009). The focal point of inception of pistachio species in the Near East incorporates the Central Asia and Turkey. Jordan positions number 13 for pistachio creation on the planet and has a world portion of 0.1 %. Creation amount of pistachio in Jordan expanded from 10 tons in 1975 to 967 tons in 2016 (FAO, 2016), with an expansion in collected zone up to 301 hectares in 2016. In vitro dust germination is a helpful strategy since it can disentangle the physiological and biochemical conditions required for the fruitful dust germination and dust tube improvement (Sanjay et al., 2016). What's more, in vitro dust germination is one of the most helpful and dependable techniques used to test the reasonability of new or put away dust. Be that as it may, dust grains of Pistacia vera L. have been viewed as hard to grow in vitro (Golan-Goldhirsh et al., 1991). Then again, dust practicality for the most part alludes to the capacity to circulate useful sperm cells to the incipient organism sac following good fertilization (Shivanna and Ram, 1993). The nature of dust is assessed based on practicality and life. All things considered, dust speaks to a basic stage in the existence pattern of plants on the grounds that suitable dust is basic for powerful multiplication of sexual plants. There has been repetitive enthusiasm for creating dependable strategies for short and long haul stockpiling of pistachio dust (Ateyyeh, 2012). Such techniques would be helpful in putting away dust to be utilized in pistachio rearing projects (Vithanage and Alexander, 1985) and supplemental fertilization programs in pistachio creation (Crane and Iwakiri, 1981) which requires assortment of adequate measures of dust and its stockpiling for short (hours to weeks) or long (months to years) time frames, while looking after suitability (Shivanna and Sawhney, 1997). What's more, putting away dust is significant for cross-fertilization, crop reproducing, physiology, biotechnology, plant biodiversity and its protection (Polito and Luza, 1988). In this examination, six pistachio cultivars were developed in Maru Agricultural Research Station, Irbid, Jordan since 1984 (all cultivars were presented from Syria). The rates of dust suitability and in vitro dust germination have never been tried in our plantation and these rates might be differed among these tried cultivars. Along these lines, the general point of this analysis was to examine in vitro germination and dust suitability in six pistachio cultivars under non-put away (new) and put away dust in fridge for one month. Likewise, this analysis was performed to discover a connection between's in vitro dust germination and suitability, to check productivity of put away dust and to figure out which pistachio cultivar could be suggested as best pollinator. In vitro dust germination and feasibility tests were completed at the biotechnology lab of the National Agricultural Research Center (NARC), Baqa'a, Jordan. Dust assortment Pollen assortment occurred during the blossoming time frame from third to sixth of April, 2018. Dust was gathered from six pistachio cultivars. Upon anthesis, pistachio bunches of every cultivar were isolates and shacked to a glass square. Care was taken through this procedure to forestall pollution. Subsequently, dust of every cultivar was put in a shut vial to be utilized later on. Dust stockpiling For every pistachio cultivar, tests of dust were set in little glass vials and these examples of non-put away (new) dust were promptly taken to research center for in vitro germination and suitability tests. Different examples were put away in fridge at 4°C for multi month to analyze in vitro dust germination and practicality with those of non-put away dust. In vitro dust germination test The medium utilized for in vitro germination testing as of late depicted by Ateyyeh (2012) contained 1% agar, 15% sucrose and 100 ppm boric corrosive (H3BO3). Dust grains were put on medium and hatched at 24°C for 24 hours. After this time, 100 dusts from every cultivar were tallied utilizing light magnifying instrument to evaluate the level of in vitro dust germination. For every cultivar, 3 imitates (petri-dishes) were utilized. Dust is viewed as sprouted if the created dust tube is surpass (2-3 times) of its width. Dust feasibility test Pollen reasonability was assessed as depicted by Ateyyeh (2012) by utilizing 1% TTC (2, 3, 5-triphenyl tetrazolium chloride) and 60% sucrose. TTC-sucrose arrangement was put away in earthy colored glass bottle in a cooler. One drop of arrangement was put onto microslide then a limited quantity of dust was suspended in the drop and spread glass was put onto the microslide, wrapped with aluminum foil and brooded in chamber room at 28°C for an hour. After hatching, 100 dusts from every cultivar were checked utilizing light magnifying lens to assess the level of dust suitability. For every cultivar, 3 recreates (microslides) were utilized. Dust grains recolored orange or brilliant red shading were viewed as suitable. Test plan and measurable examination The trial was acted in a factorial structure with six pistachio cultivars and two stockpiling medications (non-put away and put away dust for multi month).This work is partially prsented at Experts Meeting on Plant, Cellular and Molecular Biology September 10-11, 2020 Webinar" @default.
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- W3036539226 date "2020-01-01" @default.
- W3036539226 modified "2023-09-26" @default.
- W3036539226 title "EFFECT OF STORAGE TEMPERATURES AND PERIODS ON POLLEN VIABILITY AND IN VITRO GERMINATION OF PISTACHIO CULTIVARS" @default.
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