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- W3036552690 abstract "Angiogenesis plays a critical role in many physiological and pathological processes and is measured by a vascularity index derived from quantification of newly formed blood vessels. Although the identification of human endothelial cells can be accomplished by binding of antibodies against Factor VIII or binding of Ulex europeaus I agglutinin (UEA-I), in murine tissues these markers produce poor results. Since the lectin (GSA-I-B4) from the seeds of Griffonia simplicifolia which binds to -D-galactose, has been shown to bind preferentially to murine endothelial and some epithelial cells,' we determined if binding of this lectin will identify mouse endothelial cells within and around tumors growing in different organs of the mouse such as lung, kidney, bladder, and subcutaneous space. Tumors were excised, fixed in 10% formalin and processed for histology. The original procedure was modified to include a microwave antigen retrieval procedure which appears to enhance the signal better than trypsin treatment of the deparaffinized sections (Fig. 1 and 2)." @default.
- W3036552690 created "2020-06-25" @default.
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- W3036552690 date "1995-08-13" @default.
- W3036552690 modified "2023-09-27" @default.
- W3036552690 title "The use of Griffonia simplicifolia lectin, GSA-I-B-4, to determine the extent of neovascularity in murine neoplasms" @default.
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- W3036552690 doi "https://doi.org/10.1017/s0424820100141408" @default.
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