FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036554943> ?p ?o ?g. }

• W3036554943 abstract "Abstract Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme disorder in the world. Its main function is to generate NADPH that is required for anti-oxidative pathway in the cells especially in red blood cells (RBC). G6PD deficiency is X-linked and thus subject to random X-chromosome inactivation in women giving them mosaic expression of G6PD activities in their individual cells. This phenomenon makes it difficult for diagnosis with the currently available G6PD qualitative diagnostic tests. With the rolling out of newly marketed anti-malarial drug tafenoquine, which has a long half-life, screening for G6PD deficiency becomes a necessity where those with < 70% G6PD activity cannot receive this drug. Thus, evidence for a quantitative cut-off for G6PD activity is needed to ensure safe drug administration. Methods RBC models were developed to analyse the effect of oxidant on RBC oxidative markers namely total glutathione (GSH)and malondialdehyde (MDA). G6PD activity was measured using quantitative assay from Trinity Biotech and was correlated with cytofluorometric assay. RBC from two G6PD heterozygous women with different G6PD activities were also analysed for comparison. Results There was a negative correlation between G6PD activity and CuCl concentration and a strong association between G6PD activities and proportion of G6PD normal RBC in CuCl-treated models and in ex vivo RBC. However, in terms of oxidative stress markers analyses, unlike the hypothesis where the lower G6PD activity, the higher MDA and the lower GSH level, the CuCl RBC model showed that in low G6PD activities (10–30%) cells, the MDA level is lower compared to the rest of the models (p < 0.05). The ex vivo models however were in line with the hypothesis, although the result was not significant (p = 0.5). There was a significant difference between RBC with < 60% and those with > 80% G6PD activities in CuCl RBC model, but not in ex vivo RBC (p = 0.5). Genotyping heterozygous subjects showed G6PDViangchan variant with 2.97 U/gHb (33% activity) and 6.58 U/gHb (74% activity). Conclusions The GSH analysis has pointed to the 60% G6PD activity cut-off and this data is supportive of the old World Health Organization threshold for intermediate upper limit of 60% G6PD activity. However, there are significant limitations in using MDA assay with CuCl RBC model because the RBC was already stressed due to the copper treatment and thus present a different result when compared to the ex vivo model." @default.
• W3036554943 created "2020-06-25" @default.
• W3036554943 creator A5047281227 @default.
• W3036554943 creator A5051389774 @default.
• W3036554943 creator A5081882432 @default.
• W3036554943 creator A5083674453 @default.
• W3036554943 creator A5089043231 @default.
• W3036554943 date "2020-06-17" @default.
• W3036554943 modified "2023-10-15" @default.
• W3036554943 title "Determining a critical threshold for G6PD activity below which red blood cell response to oxidative stress is poor" @default.
• W3036554943 cites W121811714 @default.
• W3036554943 cites W1775749144 @default.
• W3036554943 cites W1973117081 @default.
• W3036554943 cites W1984926771 @default.
• W3036554943 cites W1994476094 @default.
• W3036554943 cites W1998536948 @default.
• W3036554943 cites W2000874885 @default.
• W3036554943 cites W2012645913 @default.
• W3036554943 cites W2014391120 @default.
• W3036554943 cites W2015375389 @default.
• W3036554943 cites W2016595227 @default.
• W3036554943 cites W2041995080 @default.
• W3036554943 cites W2044774901 @default.
• W3036554943 cites W2056993769 @default.
• W3036554943 cites W2069044914 @default.
• W3036554943 cites W2073580394 @default.
• W3036554943 cites W2081393812 @default.
• W3036554943 cites W2091362651 @default.
• W3036554943 cites W2092597744 @default.
• W3036554943 cites W2116590229 @default.
• W3036554943 cites W2119541485 @default.
• W3036554943 cites W2144876956 @default.
• W3036554943 cites W2145604426 @default.
• W3036554943 cites W2151639536 @default.
• W3036554943 cites W2164940259 @default.
• W3036554943 cites W2165056748 @default.
• W3036554943 cites W2172273789 @default.
• W3036554943 cites W2279101167 @default.
• W3036554943 cites W2281646250 @default.
• W3036554943 cites W2736571482 @default.
• W3036554943 cites W2739361295 @default.
• W3036554943 cites W2807280386 @default.
• W3036554943 doi "https://doi.org/10.1186/s12936-020-03272-y" @default.
• W3036554943 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7302344" @default.
• W3036554943 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32552815" @default.
• W3036554943 hasPublicationYear "2020" @default.
• W3036554943 type Work @default.
• W3036554943 sameAs 3036554943 @default.
• W3036554943 citedByCount "4" @default.
• W3036554943 countsByYear W30365549432021 @default.
• W3036554943 countsByYear W30365549432022 @default.
• W3036554943 countsByYear W30365549432023 @default.
• W3036554943 crossrefType "journal-article" @default.
• W3036554943 hasAuthorship W3036554943A5047281227 @default.
• W3036554943 hasAuthorship W3036554943A5051389774 @default.
• W3036554943 hasAuthorship W3036554943A5081882432 @default.
• W3036554943 hasAuthorship W3036554943A5083674453 @default.
• W3036554943 hasAuthorship W3036554943A5089043231 @default.
• W3036554943 hasBestOaLocation W30365549431 @default.
• W3036554943 hasConcept C16685009 @default.
• W3036554943 hasConcept C181199279 @default.
• W3036554943 hasConcept C203014093 @default.
• W3036554943 hasConcept C2776151105 @default.
• W3036554943 hasConcept C2776317432 @default.
• W3036554943 hasConcept C2776557347 @default.
• W3036554943 hasConcept C2778401633 @default.
• W3036554943 hasConcept C2780196906 @default.
• W3036554943 hasConcept C538909803 @default.
• W3036554943 hasConcept C55493867 @default.
• W3036554943 hasConcept C57600042 @default.
• W3036554943 hasConcept C71924100 @default.
• W3036554943 hasConcept C86803240 @default.
• W3036554943 hasConcept C98274493 @default.
• W3036554943 hasConceptScore W3036554943C16685009 @default.
• W3036554943 hasConceptScore W3036554943C181199279 @default.
• W3036554943 hasConceptScore W3036554943C203014093 @default.
• W3036554943 hasConceptScore W3036554943C2776151105 @default.
• W3036554943 hasConceptScore W3036554943C2776317432 @default.
• W3036554943 hasConceptScore W3036554943C2776557347 @default.
• W3036554943 hasConceptScore W3036554943C2778401633 @default.
• W3036554943 hasConceptScore W3036554943C2780196906 @default.
• W3036554943 hasConceptScore W3036554943C538909803 @default.
• W3036554943 hasConceptScore W3036554943C55493867 @default.
• W3036554943 hasConceptScore W3036554943C57600042 @default.
• W3036554943 hasConceptScore W3036554943C71924100 @default.
• W3036554943 hasConceptScore W3036554943C86803240 @default.
• W3036554943 hasConceptScore W3036554943C98274493 @default.
• W3036554943 hasIssue "1" @default.
• W3036554943 hasLocation W30365549431 @default.
• W3036554943 hasLocation W30365549432 @default.
• W3036554943 hasLocation W30365549433 @default.
• W3036554943 hasLocation W30365549434 @default.
• W3036554943 hasLocation W30365549435 @default.
• W3036554943 hasLocation W30365549436 @default.
• W3036554943 hasOpenAccess W3036554943 @default.
• W3036554943 hasPrimaryLocation W30365549431 @default.
• W3036554943 hasRelatedWork W2029447885 @default.
• W3036554943 hasRelatedWork W2070656560 @default.
• W3036554943 hasRelatedWork W2149003068 @default.
• W3036554943 hasRelatedWork W2325165571 @default.

• next