FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036568052> ?p ?o ?g. }

• W3036568052 endingPage "1227" @default.
• W3036568052 startingPage "1219" @default.
• W3036568052 abstract "CRISPR-Cas9 is a powerful tool for generating targeted mutations and genomic deletions. However, precise gene insertion or sequence replacement remains a major hurdle before application of CRISPR-Cas9 technology is fully realized in plant breeding. Here, we report high-frequency, selectable marker-free intra-genomic gene targeting (GT) in maize. Heat shock-inducible Cas9 was used for generating targeted double-strand breaks and simultaneous mobilization of the donor template from pre-integrated T-DNA. The construct was designed such that release of the donor template and subsequent DNA repair activated expression of the selectable marker gene within the donor locus. This approach generated up to 4.7% targeted insertion of the donor sequence into the target locus in T0 plants, with up to 86% detected donor template release and 99% mutation rate being observed at the donor loci and the genomic target site, respectively. Unlike previous in planta or intra-genomic homologous recombination reports in which the original chimeric GT plants required extensive progeny screening in the next generation to identify non-chimeric GT individuals, our method provides non-chimeric heritable GT in one generation." @default.
• W3036568052 created "2020-06-25" @default.
• W3036568052 creator A5014422072 @default.
• W3036568052 creator A5017052907 @default.
• W3036568052 creator A5019773129 @default.
• W3036568052 creator A5073126913 @default.
• W3036568052 creator A5073741172 @default.
• W3036568052 creator A5079799649 @default.
• W3036568052 creator A5090550371 @default.
• W3036568052 date "2020-08-01" @default.
• W3036568052 modified "2023-10-13" @default.
• W3036568052 title "Efficient Gene Targeting in Maize Using Inducible CRISPR-Cas9 and Marker-free Donor Template" @default.
• W3036568052 cites W1980201684 @default.
• W3036568052 cites W1988956373 @default.
• W3036568052 cites W2015015564 @default.
• W3036568052 cites W2017303675 @default.
• W3036568052 cites W2019670881 @default.
• W3036568052 cites W2037593399 @default.
• W3036568052 cites W2039375385 @default.
• W3036568052 cites W2045435533 @default.
• W3036568052 cites W2056026468 @default.
• W3036568052 cites W2064815984 @default.
• W3036568052 cites W2078308455 @default.
• W3036568052 cites W2083239972 @default.
• W3036568052 cites W2083981718 @default.
• W3036568052 cites W2085629079 @default.
• W3036568052 cites W2087918598 @default.
• W3036568052 cites W2101655911 @default.
• W3036568052 cites W2112006537 @default.
• W3036568052 cites W2112621119 @default.
• W3036568052 cites W2116041063 @default.
• W3036568052 cites W2148119081 @default.
• W3036568052 cites W2148302985 @default.
• W3036568052 cites W2149058703 @default.
• W3036568052 cites W2165016943 @default.
• W3036568052 cites W2173751778 @default.
• W3036568052 cites W2175993970 @default.
• W3036568052 cites W2259391565 @default.
• W3036568052 cites W2289813125 @default.
• W3036568052 cites W2340792251 @default.
• W3036568052 cites W2488393959 @default.
• W3036568052 cites W2518225655 @default.
• W3036568052 cites W2521332954 @default.
• W3036568052 cites W2559302185 @default.
• W3036568052 cites W2606905102 @default.
• W3036568052 cites W2738219005 @default.
• W3036568052 cites W2740956148 @default.
• W3036568052 cites W2756408218 @default.
• W3036568052 cites W2769091420 @default.
• W3036568052 cites W2789382946 @default.
• W3036568052 cites W2792937300 @default.
• W3036568052 cites W2801363769 @default.
• W3036568052 cites W2801634803 @default.
• W3036568052 cites W2804636356 @default.
• W3036568052 cites W2807550987 @default.
• W3036568052 cites W2888684269 @default.
• W3036568052 cites W2893455966 @default.
• W3036568052 cites W2900423624 @default.
• W3036568052 cites W2911206944 @default.
• W3036568052 cites W2913248883 @default.
• W3036568052 cites W2956425624 @default.
• W3036568052 cites W2966725452 @default.
• W3036568052 doi "https://doi.org/10.1016/j.molp.2020.06.008" @default.
• W3036568052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32574856" @default.
• W3036568052 hasPublicationYear "2020" @default.
• W3036568052 type Work @default.
• W3036568052 sameAs 3036568052 @default.
• W3036568052 citedByCount "55" @default.
• W3036568052 countsByYear W30365680522020 @default.
• W3036568052 countsByYear W30365680522021 @default.
• W3036568052 countsByYear W30365680522022 @default.
• W3036568052 countsByYear W30365680522023 @default.
• W3036568052 crossrefType "journal-article" @default.
• W3036568052 hasAuthorship W3036568052A5014422072 @default.
• W3036568052 hasAuthorship W3036568052A5017052907 @default.
• W3036568052 hasAuthorship W3036568052A5019773129 @default.
• W3036568052 hasAuthorship W3036568052A5073126913 @default.
• W3036568052 hasAuthorship W3036568052A5073741172 @default.
• W3036568052 hasAuthorship W3036568052A5079799649 @default.
• W3036568052 hasAuthorship W3036568052A5090550371 @default.
• W3036568052 hasBestOaLocation W30365680521 @default.
• W3036568052 hasConcept C102744134 @default.
• W3036568052 hasConcept C104317684 @default.
• W3036568052 hasConcept C111829913 @default.
• W3036568052 hasConcept C132455925 @default.
• W3036568052 hasConcept C144501496 @default.
• W3036568052 hasConcept C150194340 @default.
• W3036568052 hasConcept C17757408 @default.
• W3036568052 hasConcept C22744801 @default.
• W3036568052 hasConcept C26260077 @default.
• W3036568052 hasConcept C42411736 @default.
• W3036568052 hasConcept C54355233 @default.
• W3036568052 hasConcept C70721500 @default.
• W3036568052 hasConcept C84597430 @default.
• W3036568052 hasConcept C86803240 @default.
• W3036568052 hasConcept C98108389 @default.
• W3036568052 hasConceptScore W3036568052C102744134 @default.
• W3036568052 hasConceptScore W3036568052C104317684 @default.

• next