FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036571337> ?p ?o ?g. }

• W3036571337 abstract "The activity of KV7 channels critically contributes to the regulation of cellular electrical excitability in many cell types. In the central nervous system, the heteromeric KV7.2/KV7.3 channel is thought to be the chief molecular entity giving rise to M-currents. These K+-currents as so called because they are inhibited by the activation of Gq protein-coupled muscarinic receptors. In general, activation of Gq-protein coupled receptors (GqPCR) decrease the concentration of the phosphoinositide PI(4,5)P2 which is required for KV7 channel activity. It has been recently reported that the deactivation rate of KV7.2/KV7.3 channels decreases as a function of activation. This suggests that the open/activated channel stabilizes as activation persists. This property has been regarded as evidence for the existence of modal behavior in the activity of these channels. In particular, it has been proposed that the heteromeric KV7.2/KV7.3 channel has at least two modes of activity that can be distinguished by both their deactivation kinetics and sensitivity to retigabine. The current study was aimed at understanding the effect of PI(4,5)P2 depletion on the modal behavior of KV7.2/KV7.3 channels. Here, it was hypothesized that depleting the membrane of P(4,5)P2 would hamper the stabilization of the open/activated channel, resulting in higher rates of deactivation of the heteromeric KV7.2/KV7.3 channel. In addressing this question, it was found that the activity-dependent slowdown of the deactivation was not as prominent when channels were co-expressed with the chimeric phosphoinositide-phosphatase Ci-VS-TPIP or when cells were treated with the phosphoinositide kinase inhibitor Wortmannin. Further, it was observed that either of these approaches to deplete PI(4,5)P2 had a higher impact on the kinetic of deactivation following prolonged activation, while having little or no effect when activation was short-lived. Furthermore, it was observed that the action of either Ci-VS-TPIP or Wortmannin reduced the effect of Retigabine on the kinetics of deactivation, having a higher impact when activation was prolonged. These combined observations led to the conclusion that the deactivation kinetic of KV7.2/KV7.3 channels was sensitive to PI(4,5)P2 depletion in an activation-dependent manner, displaying a stronger effect on deactivation following prolonged activation." @default.
• W3036571337 created "2020-06-25" @default.
• W3036571337 creator A5036423818 @default.
• W3036571337 date "2020-06-19" @default.
• W3036571337 modified "2023-09-24" @default.
• W3036571337 title "Modulation of KV7 Channel Deactivation by PI(4,5)P2" @default.
• W3036571337 cites W1208303706 @default.
• W3036571337 cites W1490444160 @default.
• W3036571337 cites W1492613009 @default.
• W3036571337 cites W1499358495 @default.
• W3036571337 cites W1500064345 @default.
• W3036571337 cites W1561700273 @default.
• W3036571337 cites W1576505582 @default.
• W3036571337 cites W1583256888 @default.
• W3036571337 cites W1605503794 @default.
• W3036571337 cites W1892342982 @default.
• W3036571337 cites W1957081846 @default.
• W3036571337 cites W1963851776 @default.
• W3036571337 cites W1964381081 @default.
• W3036571337 cites W1964884778 @default.
• W3036571337 cites W1967077985 @default.
• W3036571337 cites W1969680395 @default.
• W3036571337 cites W1970279611 @default.
• W3036571337 cites W1970749986 @default.
• W3036571337 cites W1975039419 @default.
• W3036571337 cites W1982391461 @default.
• W3036571337 cites W1987349361 @default.
• W3036571337 cites W1990036972 @default.
• W3036571337 cites W1997537641 @default.
• W3036571337 cites W2000702625 @default.
• W3036571337 cites W2003420725 @default.
• W3036571337 cites W2009302038 @default.
• W3036571337 cites W2019192064 @default.
• W3036571337 cites W2025385677 @default.
• W3036571337 cites W2027104307 @default.
• W3036571337 cites W2031408824 @default.
• W3036571337 cites W2033494215 @default.
• W3036571337 cites W2034026790 @default.
• W3036571337 cites W2040299448 @default.
• W3036571337 cites W2043206871 @default.
• W3036571337 cites W2048894410 @default.
• W3036571337 cites W2052082800 @default.
• W3036571337 cites W2053120786 @default.
• W3036571337 cites W2054750757 @default.
• W3036571337 cites W2056758436 @default.
• W3036571337 cites W2059690890 @default.
• W3036571337 cites W2061062706 @default.
• W3036571337 cites W2061170492 @default.
• W3036571337 cites W2061523258 @default.
• W3036571337 cites W2062463079 @default.
• W3036571337 cites W2063113128 @default.
• W3036571337 cites W2066278737 @default.
• W3036571337 cites W2070032514 @default.
• W3036571337 cites W2072877245 @default.
• W3036571337 cites W2079373480 @default.
• W3036571337 cites W2081126867 @default.
• W3036571337 cites W2081945152 @default.
• W3036571337 cites W2087233406 @default.
• W3036571337 cites W2090469070 @default.
• W3036571337 cites W2090619430 @default.
• W3036571337 cites W2091884933 @default.
• W3036571337 cites W2094011065 @default.
• W3036571337 cites W2094902307 @default.
• W3036571337 cites W2095460941 @default.
• W3036571337 cites W2097411147 @default.
• W3036571337 cites W2103687341 @default.
• W3036571337 cites W2104682405 @default.
• W3036571337 cites W2104759180 @default.
• W3036571337 cites W2107330391 @default.
• W3036571337 cites W2111187306 @default.
• W3036571337 cites W2112922820 @default.
• W3036571337 cites W2118025511 @default.
• W3036571337 cites W2122652505 @default.
• W3036571337 cites W2131842347 @default.
• W3036571337 cites W2132352566 @default.
• W3036571337 cites W2137263523 @default.
• W3036571337 cites W2137872660 @default.
• W3036571337 cites W2138022345 @default.
• W3036571337 cites W2140894987 @default.
• W3036571337 cites W2142318256 @default.
• W3036571337 cites W2147391231 @default.
• W3036571337 cites W2155770440 @default.
• W3036571337 cites W2158977304 @default.
• W3036571337 cites W2159403877 @default.
• W3036571337 cites W2159579442 @default.
• W3036571337 cites W2161033576 @default.
• W3036571337 cites W2161145612 @default.
• W3036571337 cites W2162855810 @default.
• W3036571337 cites W2166707375 @default.
• W3036571337 cites W2171553829 @default.
• W3036571337 cites W2273364344 @default.
• W3036571337 cites W2278978875 @default.
• W3036571337 cites W2318319321 @default.
• W3036571337 cites W2346783774 @default.
• W3036571337 cites W2417477273 @default.
• W3036571337 cites W2418454736 @default.
• W3036571337 cites W2467239676 @default.
• W3036571337 cites W2516134676 @default.
• W3036571337 cites W2527174369 @default.
• W3036571337 cites W2559366165 @default.

• next