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- W3036636119 abstract "In previous experiments we demonstrated that 4, 12 and 24 weeks after rat adrenal cortex autotransplantation, the animals survived and the autotransplant was easily reproducible. It was observed that 4 weeks after transplantation the plasma corticosterone concentration (0.20 μg/ml) was already comparable to that in controls and that the light and electron microscopy features of the cells of the glomerulosa and fasciculata zones were similar to those of adrenal cells in control animals. In order to clarify the origin of glandular cells in the autotransplanted animals, autoradiographic experiments were made. Four male rats with body weights ranging from 180to195 gm underwent bilateral adrenalectomy via two dorsal incisions made along the lateral border of the dorsal muscle mass. Pieces of adrenals were autotransplanted immediately under the skin of the dorsal region. Four animals were “sham-operated” without removal of adrenals and served as controls. The animals were fed “ad libitum” and with saline solution, and were sacrificed at 2,3,7 and 15 days. 3 H-Thymidine (1 μCi/gm body weight) was administrated intraperitoneally to each animal two hours before the sacrifice. Necropsy was carried out in all animals in order to search for accessory adrenals. The autotransplanted pieces were removed, fixed and processed for LM and EM morphologic and autoradiographic studies. For LM autoradiographic studies Ilford K 5 nuclear emulsion was used, and the sections exposed for 33 days; for EM autoradiographic studies Ilford L 4 nuclear emulsion was used, the sections exposed for 90 days, and the radio autographs developed with Kodak D 19b." @default.
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- W3036636119 date "1990-08-12" @default.
- W3036636119 modified "2023-10-18" @default.
- W3036636119 title "Autotransplantation of adrenal cortex: An autoradiographic study" @default.
- W3036636119 doi "https://doi.org/10.1017/s0424820100159175" @default.
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