FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036647629> ?p ?o ?g. }

• W3036647629 endingPage "1863" @default.
• W3036647629 startingPage "1855" @default.
• W3036647629 abstract "l-Proline takes a significant role in the pharmaceutical and chemical industries as well as graziery. Typical biosynthesis of l-proline is from l-glutamate, involving three enzyme reactions as well as a spontaneous cyclization. Alternatively, l-proline can be also synthesized in l-ornithine and/or l-arginine producing strains by an ornithine aminotransferase (OCD). In this study, a strategy of directed evolution combining rare codon selection and pEvolvR was developed to screen OCD with high catalytic efficiency, improving l-proline production from l-arginine chassis cells. The mutations were generated by CRISPR-assisted DNA polymerases and were screened by growth-coupled rare codon selection system. OCDK205G/M86K/T162A from Pseudomonas putida was identified with 2.85-fold increase in catalytic efficiency for the synthesis of l-proline. Furthermore, we designed and optimized RBS for the BaargI and Ppocd coupling cascade using RedLibs, as well as sRNA inhibition of argF to moderate l-proline biosynthesis in l-arginine overproducing Corynebacterium crenatum. The strain PS6 with best performance reached 15.3 g/L l-proline in the shake flask and showed a titer of 38.4 g/L in a 5 L fermenter with relatively low concentration of residual l-ornithine and/or l-arginine." @default.
• W3036647629 created "2020-06-25" @default.
• W3036647629 creator A5002711264 @default.
• W3036647629 creator A5037146416 @default.
• W3036647629 creator A5045322000 @default.
• W3036647629 creator A5058791842 @default.
• W3036647629 creator A5074999356 @default.
• W3036647629 creator A5075412523 @default.
• W3036647629 creator A5077886604 @default.
• W3036647629 creator A5084154791 @default.
• W3036647629 creator A5086877829 @default.
• W3036647629 date "2020-06-18" @default.
• W3036647629 modified "2023-10-13" @default.
• W3036647629 title "Directed Evolution of Ornithine Cyclodeaminase Using an EvolvR-Based Growth-Coupling Strategy for Efficient Biosynthesis of <scp>l</scp>-Proline" @default.
• W3036647629 cites W127120502 @default.
• W3036647629 cites W1457545537 @default.
• W3036647629 cites W1518883964 @default.
• W3036647629 cites W1876562917 @default.
• W3036647629 cites W1937300716 @default.
• W3036647629 cites W1964142551 @default.
• W3036647629 cites W1968984443 @default.
• W3036647629 cites W2001339406 @default.
• W3036647629 cites W2005931802 @default.
• W3036647629 cites W2035418696 @default.
• W3036647629 cites W2043917282 @default.
• W3036647629 cites W2047360542 @default.
• W3036647629 cites W2086462671 @default.
• W3036647629 cites W2090733444 @default.
• W3036647629 cites W2103304356 @default.
• W3036647629 cites W2104151224 @default.
• W3036647629 cites W2127830286 @default.
• W3036647629 cites W2135572188 @default.
• W3036647629 cites W2140373206 @default.
• W3036647629 cites W2148641266 @default.
• W3036647629 cites W2158076215 @default.
• W3036647629 cites W2161042136 @default.
• W3036647629 cites W2164088236 @default.
• W3036647629 cites W2169840290 @default.
• W3036647629 cites W2314251954 @default.
• W3036647629 cites W2315890352 @default.
• W3036647629 cites W2495240111 @default.
• W3036647629 cites W2561771945 @default.
• W3036647629 cites W2618002610 @default.
• W3036647629 cites W2728777258 @default.
• W3036647629 cites W2744248965 @default.
• W3036647629 cites W2757119213 @default.
• W3036647629 cites W2762652086 @default.
• W3036647629 cites W2799967732 @default.
• W3036647629 cites W2810513166 @default.
• W3036647629 cites W2887001543 @default.
• W3036647629 cites W2889233581 @default.
• W3036647629 cites W2910618174 @default.
• W3036647629 cites W2911769745 @default.
• W3036647629 cites W2954191972 @default.
• W3036647629 cites W2969819767 @default.
• W3036647629 doi "https://doi.org/10.1021/acssynbio.0c00198" @default.
• W3036647629 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32551572" @default.
• W3036647629 hasPublicationYear "2020" @default.
• W3036647629 type Work @default.
• W3036647629 sameAs 3036647629 @default.
• W3036647629 citedByCount "20" @default.
• W3036647629 countsByYear W30366476292021 @default.
• W3036647629 countsByYear W30366476292022 @default.
• W3036647629 countsByYear W30366476292023 @default.
• W3036647629 crossrefType "journal-article" @default.
• W3036647629 hasAuthorship W3036647629A5002711264 @default.
• W3036647629 hasAuthorship W3036647629A5037146416 @default.
• W3036647629 hasAuthorship W3036647629A5045322000 @default.
• W3036647629 hasAuthorship W3036647629A5058791842 @default.
• W3036647629 hasAuthorship W3036647629A5074999356 @default.
• W3036647629 hasAuthorship W3036647629A5075412523 @default.
• W3036647629 hasAuthorship W3036647629A5077886604 @default.
• W3036647629 hasAuthorship W3036647629A5084154791 @default.
• W3036647629 hasAuthorship W3036647629A5086877829 @default.
• W3036647629 hasConcept C181199279 @default.
• W3036647629 hasConcept C185592680 @default.
• W3036647629 hasConcept C2775895851 @default.
• W3036647629 hasConcept C2776796294 @default.
• W3036647629 hasConcept C2777468819 @default.
• W3036647629 hasConcept C2779815552 @default.
• W3036647629 hasConcept C2780543182 @default.
• W3036647629 hasConcept C515207424 @default.
• W3036647629 hasConcept C553450214 @default.
• W3036647629 hasConcept C55493867 @default.
• W3036647629 hasConcept C86803240 @default.
• W3036647629 hasConceptScore W3036647629C181199279 @default.
• W3036647629 hasConceptScore W3036647629C185592680 @default.
• W3036647629 hasConceptScore W3036647629C2775895851 @default.
• W3036647629 hasConceptScore W3036647629C2776796294 @default.
• W3036647629 hasConceptScore W3036647629C2777468819 @default.
• W3036647629 hasConceptScore W3036647629C2779815552 @default.
• W3036647629 hasConceptScore W3036647629C2780543182 @default.
• W3036647629 hasConceptScore W3036647629C515207424 @default.
• W3036647629 hasConceptScore W3036647629C553450214 @default.
• W3036647629 hasConceptScore W3036647629C55493867 @default.
• W3036647629 hasConceptScore W3036647629C86803240 @default.
• W3036647629 hasFunder F4320321001 @default.
• W3036647629 hasFunder F4320321106 @default.

• next