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- W3036657104 abstract "It is now possible to work with wet materials by using environmental chambers. Biologists would like to evaluate the advantages of this development. The unknowns are: (a) the difficulty of constructing the equipment and it's commercial availability, (b) the problems in preparing wet specimens and viewing them, (c) the resolution and contrast available (d) the difficulties peculiar to wet specimens and (e) the advantages of wet over frozen specimens. Finally, there remains the question - can living cells be examined? This talk will attempt to answer these questions. Routine wet electron microscopy has followed the introduction of differentially pumped chambers in place of film sealed chambers. Electron diffraction patterns of wet protein crystals (Fig. 1) provide a sensitive test of the performance of hydration chambers. If the vapor pressure drops more than 10% below saturation the pattern disappears due to the disorganization of drying." @default.
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- W3036657104 date "1975-01-01" @default.
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- W3036657104 title "What Improvement in Electron Microscopy and Electron Diffraction Results from Using Wet Biological Materials?" @default.
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- W3036657104 doi "https://doi.org/10.1017/s0424820100115109" @default.
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