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- W3036673313 abstract "Nonspecific calcinosis engendered by dihydrotachysterol ( DHT ) is associated with massive extraosseous calcium deposits, in connection with which acid mucopolysaccharides ( AM ) can be observed. Cardiac muscle and the vascular system are particularly affected. The acri-flavine-phosphotungsten acid ( PTA ) contrast-method of tracing AM is based on the affinity of acriflavine for basophile material in the tissue. An electron dense contrast is achieved by means of the complex of acriflavine, which is selectively bound in the tissue, and PTA. Six month old albino rats were sacrificed 1, 2, 3, 5, 8, 10, 13, 17 and 20 days after per os-administration of 3. 0 mg DHT. Tissue samples from cardiac muscle and aorta were first fixed in 2 % glutaraldehyde phosphate-buffer, pH 7. 0 for two hours and then pre-incubated in darkness with 2. 0 x 10 -2 M acriflavine in 50 % ethanol phosphate-buffer overnight." @default.
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- W3036673313 date "1978-08-01" @default.
- W3036673313 modified "2023-09-26" @default.
- W3036673313 title "Electron microscope tracing of acid mucopolysaccharides after administration of dihydrotachysterol" @default.
- W3036673313 doi "https://doi.org/10.1017/s0424820100084065" @default.
- W3036673313 hasPublicationYear "1978" @default.
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