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- W3036683476 abstract "Although hepatic sinusoidal alterations have often been observed in AIDS patients, the infection of the EC of the liver with HIV has never been accounted for. The present study aims at demonstrating that EC isolated from the hepatic sinusoid may be permissive for HIV-1. EC were isolated from surgical biopsies obtained from HIV-seronegative patients suffering from liver cancer. The cells were prepared by collagenase dissociation of the tissue and centrifugal elutriation. The cells were cultured in 24-well culture plates in the medium previously described for 5-6 days before being infected with 2.10 4 syncytia-forming units of HIV-1 (isolate HTLV-III B) per well. They were fixed after 7, 14 and 19 days of infection and routinely prepared for TEM and SEM. The presence in the EC of the von Willebrand factor was tested by immunofluorescence (FVIII.rAg, Behring). Typical syncytia resulting from the infection were observed (Figs. 1, 2), their endothelial origin being demonstrated by the presence of the von Willebrand factor (Fig. 1). This origin could be further assessed by observing characteristic fenestrae (Figs. 3, 4) and Weibel-Palade bodies (Fig. 5)." @default.
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- W3036683476 date "1990-08-12" @default.
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- W3036683476 title "Ultrastructural evidence of Human Immunodeficiency Virus (HIV-1) multiplication in cultured endothelial cells (EC) of the human hepatic sinusoid" @default.
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- W3036683476 doi "https://doi.org/10.1017/s0424820100159278" @default.
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