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- W3036684447 abstract "Growing interest in the health benefits of soy isoflavones has led to research in the isolation of individual isoflavone species for further application. Herein, we develop a new strategy to isolate daidzein, genistein, daidzin and genistin in soybean. We investigated the impact of solvents used and the extraction time on the extracted isoflavone contents from soybean. A 30-min extraction with 65% aqueous methanol gave a total isoflavone yield of 345 mg/100 g soybean, the highest value among tested conditions. Further, we proposed a two-stage adsorption/desorption chromatography comprising macroporous resin and aluminium oxide to isolate isoflavone. First, HP-20 resin was used to separate the glucosidic and aglyconic forms of isoflavone, then individual species of isoflavone could be isolated using aluminium oxide by specific retention of 5-hydroxy isoflavone. This process achieved overall high recovery (82–97%) and purity (92–95%) of the four isoflavones, which confirms a high separating efficiency for isoflavones from soybean." @default.
- W3036684447 created "2020-06-25" @default.
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- W3036684447 date "2020-11-01" @default.
- W3036684447 modified "2023-10-04" @default.
- W3036684447 title "Isolation of individual isoflavone species from soybean by solvent extraction followed by the combination of macroporous resin and aluminium oxide separation" @default.
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- W3036684447 doi "https://doi.org/10.1016/j.foodchem.2020.127312" @default.
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