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- W3036686044 abstract "Mature human dental enamel has presented investigators with several difficulties in ultramicrotomy of specimens for electron microscopy due to its high degree of mineralization. This study explores the possibility of combining ion-milling and high voltage electron microscopy as a means of circumventing the problems of ultramicrotomy. A longitudinal section of an extracted human third molar was ground to a thickness of about 30 um and polarized light micrographs were taken. The specimen was attached to a single hole grid and thinned by argon-ion bombardment at 15° incidence while rotating at 15 rpm. The beam current in each of two guns was 50 μA with an accelerating voltage of 4 kV. A 20 nm carbon coating was evaporated onto the specimen to prevent an electron charge from building up during electron microscopy." @default.
- W3036686044 created "2020-06-25" @default.
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- W3036686044 date "1974-08-15" @default.
- W3036686044 modified "2023-09-23" @default.
- W3036686044 title "High Voltage Electron Microscopy of Ion-Milled Dental Enamel" @default.
- W3036686044 doi "https://doi.org/10.1017/s0424820100050317" @default.
- W3036686044 hasPublicationYear "1974" @default.
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