Matches in SemOpenAlex for { <https://semopenalex.org/work/W3036690941> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W3036690941 endingPage "463" @default.
- W3036690941 startingPage "462" @default.
- W3036690941 abstract "The three-dimensional organization of cytoskeletal components in the early Drosophila melanogaster embryos during cellularization was examined by confocal laser scanning microscopy of the whole embryos and thin section electron microscopy. For confocal microscopy, Drosophila embryos at 2-3 hr after egg-laying were dechorionated and fixed with 8% paraformaldehyde-1% picric acid in 0.1M phosphate buffer (pH 7.2). Embryos were first blocked with normal goat serum, incubated with monoclonal antibody raised against Drosophila embryo α-tubulin for 4 hr, and then were incubated with FITC-conjugated anti-mouse IgG for 2 hr. Some embryos were stained with rhodamine-labeled phailoidin for F-actin visualization. After staining, the whole embryos were mounted on slide glass with an appropriate spacer and examined under the confocal microscope (Bio-Rad, Lasersharp MRC-500). For electron microscopy, dechorionated embryos were fixed with 1/2 Karnovsky's fixative followed by OsO 4 fixation. To better preserve actin filaments, embryos were fixed with the same 1/2 Karnovsky's fixative containing 10 μM phailoidin and 0.1% saponin in 0.1M phosphate buffer, pH 7.2. Such fixed embryos were dehydrated and then embedded in Epoxy resin. Thin sections were cut and examined under a Hitachi H-800 type electron microscope." @default.
- W3036690941 created "2020-06-25" @default.
- W3036690941 creator A5015054549 @default.
- W3036690941 creator A5034253304 @default.
- W3036690941 date "1990-08-12" @default.
- W3036690941 modified "2023-09-25" @default.
- W3036690941 title "Organization of the cytoskeleton during cellularization in Drosophila melanogaster embryos: Confocal laser scanning and electron microscopy" @default.
- W3036690941 doi "https://doi.org/10.1017/s0424820100159850" @default.
- W3036690941 hasPublicationYear "1990" @default.
- W3036690941 type Work @default.
- W3036690941 sameAs 3036690941 @default.
- W3036690941 citedByCount "0" @default.
- W3036690941 crossrefType "journal-article" @default.
- W3036690941 hasAuthorship W3036690941A5015054549 @default.
- W3036690941 hasAuthorship W3036690941A5034253304 @default.
- W3036690941 hasConcept C105702510 @default.
- W3036690941 hasConcept C109986877 @default.
- W3036690941 hasConcept C120665830 @default.
- W3036690941 hasConcept C121332964 @default.
- W3036690941 hasConcept C12554922 @default.
- W3036690941 hasConcept C136009344 @default.
- W3036690941 hasConcept C142669718 @default.
- W3036690941 hasConcept C142724271 @default.
- W3036690941 hasConcept C147080431 @default.
- W3036690941 hasConcept C1491633281 @default.
- W3036690941 hasConcept C153911025 @default.
- W3036690941 hasConcept C159985019 @default.
- W3036690941 hasConcept C178790620 @default.
- W3036690941 hasConcept C185592680 @default.
- W3036690941 hasConcept C190062978 @default.
- W3036690941 hasConcept C192562407 @default.
- W3036690941 hasConcept C196843134 @default.
- W3036690941 hasConcept C2524010 @default.
- W3036690941 hasConcept C26771246 @default.
- W3036690941 hasConcept C2778723968 @default.
- W3036690941 hasConcept C2779178360 @default.
- W3036690941 hasConcept C2780848204 @default.
- W3036690941 hasConcept C33923547 @default.
- W3036690941 hasConcept C54355233 @default.
- W3036690941 hasConcept C55493867 @default.
- W3036690941 hasConcept C71924100 @default.
- W3036690941 hasConcept C74864618 @default.
- W3036690941 hasConcept C86803240 @default.
- W3036690941 hasConcept C87555872 @default.
- W3036690941 hasConcept C93877712 @default.
- W3036690941 hasConcept C95444343 @default.
- W3036690941 hasConceptScore W3036690941C105702510 @default.
- W3036690941 hasConceptScore W3036690941C109986877 @default.
- W3036690941 hasConceptScore W3036690941C120665830 @default.
- W3036690941 hasConceptScore W3036690941C121332964 @default.
- W3036690941 hasConceptScore W3036690941C12554922 @default.
- W3036690941 hasConceptScore W3036690941C136009344 @default.
- W3036690941 hasConceptScore W3036690941C142669718 @default.
- W3036690941 hasConceptScore W3036690941C142724271 @default.
- W3036690941 hasConceptScore W3036690941C147080431 @default.
- W3036690941 hasConceptScore W3036690941C1491633281 @default.
- W3036690941 hasConceptScore W3036690941C153911025 @default.
- W3036690941 hasConceptScore W3036690941C159985019 @default.
- W3036690941 hasConceptScore W3036690941C178790620 @default.
- W3036690941 hasConceptScore W3036690941C185592680 @default.
- W3036690941 hasConceptScore W3036690941C190062978 @default.
- W3036690941 hasConceptScore W3036690941C192562407 @default.
- W3036690941 hasConceptScore W3036690941C196843134 @default.
- W3036690941 hasConceptScore W3036690941C2524010 @default.
- W3036690941 hasConceptScore W3036690941C26771246 @default.
- W3036690941 hasConceptScore W3036690941C2778723968 @default.
- W3036690941 hasConceptScore W3036690941C2779178360 @default.
- W3036690941 hasConceptScore W3036690941C2780848204 @default.
- W3036690941 hasConceptScore W3036690941C33923547 @default.
- W3036690941 hasConceptScore W3036690941C54355233 @default.
- W3036690941 hasConceptScore W3036690941C55493867 @default.
- W3036690941 hasConceptScore W3036690941C71924100 @default.
- W3036690941 hasConceptScore W3036690941C74864618 @default.
- W3036690941 hasConceptScore W3036690941C86803240 @default.
- W3036690941 hasConceptScore W3036690941C87555872 @default.
- W3036690941 hasConceptScore W3036690941C93877712 @default.
- W3036690941 hasConceptScore W3036690941C95444343 @default.
- W3036690941 hasIssue "3" @default.
- W3036690941 hasLocation W30366909411 @default.
- W3036690941 hasOpenAccess W3036690941 @default.
- W3036690941 hasPrimaryLocation W30366909411 @default.
- W3036690941 hasRelatedWork W1984789941 @default.
- W3036690941 hasRelatedWork W2039516224 @default.
- W3036690941 hasRelatedWork W2082895746 @default.
- W3036690941 hasRelatedWork W2115915405 @default.
- W3036690941 hasRelatedWork W2354314765 @default.
- W3036690941 hasRelatedWork W2411673947 @default.
- W3036690941 hasRelatedWork W2560572525 @default.
- W3036690941 hasRelatedWork W3036757773 @default.
- W3036690941 hasRelatedWork W3046519579 @default.
- W3036690941 hasRelatedWork W3154593472 @default.
- W3036690941 hasVolume "48" @default.
- W3036690941 isParatext "false" @default.
- W3036690941 isRetracted "false" @default.
- W3036690941 magId "3036690941" @default.
- W3036690941 workType "article" @default.