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- W3036691738 endingPage "853.e11" @default.
- W3036691738 startingPage "836" @default.
- W3036691738 abstract "The resolution and dimensionality with which biologists can characterize cell types have expanded dramatically in recent years, and intersectional consideration of such features (e.g., multiple gene expression and anatomical parameters) is increasingly understood to be essential. At the same time, genetically targeted technology for writing in and reading out activity patterns for cells in living organisms has enabled causal investigation in physiology and behavior; however, cell-type-specific delivery of these tools (including microbial opsins for optogenetics and genetically encoded Ca2+ indicators) has thus far fallen short of versatile targeting to cells jointly defined by many individually selected features. Here, we develop a comprehensive intersectional targeting toolbox including 39 novel vectors for joint-feature-targeted delivery of 13 molecular payloads (including opsins, indicators, and fluorophores), systematic approaches for development and optimization of new intersectional tools, hardware for in vivo monitoring of expression dynamics, and the first versatile single-virus tools (Triplesect) that enable targeting of triply defined cell types." @default.
- W3036691738 created "2020-06-25" @default.
- W3036691738 creator A5004786569 @default.
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- W3036691738 creator A5056572514 @default.
- W3036691738 creator A5067787994 @default.
- W3036691738 creator A5077768705 @default.
- W3036691738 date "2020-09-01" @default.
- W3036691738 modified "2023-10-13" @default.
- W3036691738 title "Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells of Behaving Mammals" @default.
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